Chung Julia, Sepunaru Lior, Plaxco Kevin W
Interdepartmental Program in Biomedical Science and Engineering, University of California at Santa Barbara, Santa Barbara, California 93106, USA.
Department of Chemistry and Biochemistry, University of California at Santa Barbara, Santa Barbara, California 93106, USA.
ECS Sens Plus. 2022 Mar;1(1). doi: 10.1149/2754-2726/ac60b2. Epub 2022 Apr 13.
Electrochemical aptamer-based (EAB) sensors encompass the only biosensor approach yet reported that is simultaneously: (1) independent of the chemical or enzymatic reactivity of its target, rendering it general; (2) continuous and real-time; and (3) selective enough to deploy in the living body. Consistent with this, EAB sensors supporting the seconds-resolved, real-time measurement of multiple drugs and metabolites have been reported, suggesting the approach may prove of value in biomedical research and the diagnosis, treatment, and monitoring of disease. However, to apply these devices in long-duration animal models, much less in human patients, requires that they be free of any significant pathogen load. Thus motivated, here we have characterized the compatibility of EAB sensors with standard sterilization and high-level disinfection techniques. Doing so, we find that, while many lead to significant sensor degradation, treatment with CIDEX OPA (0.55% phthalaldehyde) leads to effective disinfection without causing any detectable loss in sensor performance.
基于电化学适配体的（EAB）传感器是目前已报道的唯一一种同时具备以下特性的生物传感器方法：（1）不依赖其靶标的化学或酶促反应活性，具有通用性；（2）可连续实时监测；（3）选择性足以在活体中应用。与此相符的是，已有报道称EAB传感器可支持对多种药物和代谢物进行秒级分辨的实时测量，这表明该方法在生物医学研究以及疾病的诊断、治疗和监测中可能具有价值。然而，要将这些设备应用于长期动物模型，更不用说应用于人类患者，就要求它们没有任何显著的病原体负载。因此，我们在此对EAB传感器与标准灭菌和高水平消毒技术的兼容性进行了表征。通过这样做，我们发现，虽然许多方法会导致传感器显著降解，但用凯得牌邻苯二甲醛（0.55%邻苯二甲醛）处理可实现有效消毒，且不会导致传感器性能出现任何可检测到的损失。
