鉴定和分析人巨细胞病毒编码的环状 RNA。
Identification and characterization of human cytomegalovirus-encoded circular RNAs.
机构信息
Department of Microorganism Laboratory, Shenyang Center for Disease Control and Prevention, Shenyang, China.
Virology Laboratory, Shengjing Hospital of China Medical University, Shenyang, China.
出版信息
Front Cell Infect Microbiol. 2022 Nov 14;12:980974. doi: 10.3389/fcimb.2022.980974. eCollection 2022.
Circular RNA (circRNA) exists extensively and plays essential roles in serving as microRNA (miRNA) or protein sponges and protein scaffolding in many organisms. However, the profiles and potential functions of the virus-encoded circRNA, including human cytomegalovirus (HCMV)-encoded circular RNAs, remain unclear. In the present study, HCMV-encoded circRNAs profile in human embryonic lung fibroblasts (HELF) with lytic infection was investigated using RNA deep sequencing and bioinformatics analysis. In total, 629 HCMV-encoded circRNAs were identified with various expression patterns in our results. The full sequences and alternative splicings of circUS12, circUL55, and circUL89 were verified by reverse transcriptase-PCR (RT-PCR) with divergent primers followed and Sanger sequencing. Transcription of circUL89 was validated by Northern blot. The HCMV-encoded circRNA-miRNA network analyses revealed the potential function of HCMV-encoded circRNAs during HCMV infection in HELFs. Collectively, HCMV infection deduced abundant HCMV-associated circRNAs during infection, and the HCMV-encoded circRNAs might play important roles in benefiting HCMV infection.
环状 RNA（circRNA）广泛存在，并在许多生物体中作为 microRNA（miRNA）或蛋白质海绵和蛋白质支架发挥重要作用。然而，病毒编码的 circRNA 的特征和潜在功能，包括人类巨细胞病毒（HCMV）编码的环状 RNA，仍然不清楚。在本研究中，使用 RNA 深度测序和生物信息学分析研究了具有裂解感染的人胚胎肺成纤维细胞（HELF）中的 HCMV 编码的 circRNA 谱。在我们的结果中，总共鉴定出 629 种具有不同表达模式的 HCMV 编码 circRNA。通过使用反转录酶-PCR（RT-PCR）和 Sanger 测序用不同的引物验证了 circUS12、circUL55 和 circUL89 的全长序列和可变剪接。通过 Northern blot 验证了 circUL89 的转录。HCMV 编码的 circRNA-miRNA 网络分析揭示了 HCMV 编码的 circRNA 在 HELF 中的 HCMV 感染过程中的潜在功能。总的来说，HCMV 感染在感染过程中推断出大量与 HCMV 相关的 circRNA，而 HCMV 编码的 circRNA 可能在有利于 HCMV 感染方面发挥重要作用。
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