College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.
Appl Microbiol Biotechnol. 2023 Jan;107(1):9-23. doi: 10.1007/s00253-022-12315-0. Epub 2022 Dec 2.
Enzymes, as elements with catalytic functions, can be rationally designed and multiple assembled to form a composite catalytic system and achieve cascade catalytic functions. Enzyme cascade catalysis could produce various chemical products with high conversion rate in short time. With the development of DNA nanotechnology, assembling enzymes to different nucleic acid-based scaffolds in different spatial organizations could effectively improve the catalytic efficiency of enzymes. Herein, we review the construction and application of nucleic acid-based scaffold systems from the perspective of template assembly in three dimensions. The challenges and future outlooks in the development of enzyme cascades are also discussed. KEY POINTS: • The principles and construction of various nucleic acid scaffolds are summarized • The application of nucleic acid scaffolds in enzyme cascade catalysis is discussed.
酶作为具有催化功能的元件，可以进行合理设计和多次组装，形成复合催化体系，实现级联催化功能。酶级联催化可以在短时间内以高转化率生产各种化学产品。随着 DNA 纳米技术的发展，将酶组装到不同的空间组织的基于核酸的支架上，可以有效地提高酶的催化效率。本文从三维模板组装的角度综述了基于核酸的支架系统的构建和应用。还讨论了酶级联发展中面临的挑战和未来展望。
总结了各种核酸支架的原理和构建
讨论了核酸支架在酶级联催化中的应用
