Yun Yanhong, Shi Huiyu, Wang Yanyu, Yang Fengyuan, Zhang Yuanxin, Feng Haibo, Chen Junpu, Wang Xuemei
College of Animal Science and Technology, Hainan University, Haikou 570228, China.
Animals (Basel). 2023 Apr 19;13(8):1401. doi: 10.3390/ani13081401.
The purple tubers of L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of L. and its crude extracts on cells prior to oxidative stress. To establish a suitable oxidative damage model, an injured model of IPEC-J2 cells was created using HO as the oxidant. Specifically, when the concentration of HO was 120 μmol/L and the injured time was 8 h, the survival rate of cells decreased to approximately 70%, and the cells exhibited a noticeable oxidative stress reaction. Moreover, the crude extracts of L. demonstrated beneficial pre-protective effects on IPEC-J2 cells by increasing the total antioxidant capacity (T-AOC) and catalase (CAT) activities, augmenting the expression of total superoxide dismutase (T-SOD) and its genes, reducing the content of malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-P) and its expression of genes, and promoting the expression of glucose transporter SGLT1 gene while reducing that of GULT2 gene, thereby facilitating the entry of anthocyanins into cells. In addition, the 50 μg/mL crude extracts effectively inhibited the phosphorylation of IκB and the p65 protein, thus reducing cellular oxidative stress. Given these findings, L. can be considered a natural antioxidant for practical breeding and production purposes, with an optimal concentration of crude extracts in this experiment being 50 μg/mL.
已发现L.的紫色块茎含有多种生物活性化学成分，包括花青素，这使得研究L.及其粗提物在氧化应激之前对细胞的预保护作用具有重要意义。为建立合适的氧化损伤模型，以HO作为氧化剂建立了IPEC-J2细胞损伤模型。具体而言，当HO浓度为120μmol/L且损伤时间为8小时时，细胞存活率降至约70%，并且细胞表现出明显的氧化应激反应。此外，L.的粗提物对IPEC-J2细胞具有有益的预保护作用，可提高总抗氧化能力（T-AOC）和过氧化氢酶（CAT）活性，增强总超氧化物歧化酶（T-SOD）及其基因的表达，降低丙二醛（MDA）含量以及谷胱甘肽过氧化物酶（GSH-P）活性及其基因表达，并促进葡萄糖转运蛋白SGLT1基因的表达，同时降低GULT2基因的表达，从而促进花青素进入细胞。此外，50μg/mL的粗提物有效抑制IκB和p65蛋白的磷酸化，从而减轻细胞氧化应激。基于这些发现，L.可被视为用于实际育种和生产目的的天然抗氧化剂，本实验中粗提物的最佳浓度为50μg/mL。
