Laboratory of Technological Innovation in Health (LAIS), Hospital Universitário Onofre Lopes, Federal University of Rio Grande do Norte (UFRN), Natal 59078-970, Brazil.
Health Sciences Research Unit: Nursing (UICISA:E), The Nursing School of Coimbra (ESEnfC), 3004-011 Coimbra, Portugal.
Int J Environ Res Public Health. 2022 Dec 3;19(23):16206. doi: 10.3390/ijerph192316206.
The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0') or 15 min (T15') after incubation, indicating its potential use as a point-of-care method as a screening strategy.
实验室诊断的改进是减少全球梅毒病例的关键步骤。在本文中，我们提出了一种基于阻抗的方法来检测抗原和抗体，作为梅毒实验室诊断的辅助工具。我们评估了在存在或不存在聚-L-赖氨酸的情况下，在碳或金纳米粒子修饰的碳电极孵育后获得的伏安信号。我们的结果表明，从电极获得的信号足以立即（T0'）或孵育 15 分钟后（T15'）区分感染和未感染的样本，表明其作为即时检测方法作为一种筛选策略的潜在用途。
