Li Man, Li Yiman, Zhang Wendong, Li Shuhao, Gao Yong, Ai Xizhen, Zhang Dalong, Liu Binbin, Li Qingming
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, 271018, China; State Key Laboratory of Crop Biology, Tai'an, Shandong, 271018, China.
Anal Biochem. 2018 Oct 15;559:71-85. doi: 10.1016/j.ab.2018.08.020. Epub 2018 Aug 25.
Elevated atmospheric CO alleviates moderate to severe drought stresses at physiological level in cucumber. To investigate the underlying metabolic mechanisms, cucumber seedlings were treated with two [CO] and three water treatments combinations, and their leaves were analyzed using a non-targeted metabolomics approach. The results showed that elevated [CO] changed 79 differential metabolites which were mainly associated with alanine, aspartate and glutamate metabolism; arginine and proline metabolism; TCA cycle; and glycerophospholipid metabolism under moderate drought stress. Moreover, elevated [CO] promoted the accumulation of secondary metabolites; including isoferulic acid, m-coumaric acid and salicyluric acid. Under severe drought stress, elevated [CO] changed 26 differential metabolites which mainly involved in alanine, aspartate and glutamate metabolism; pyruvate metabolism; arginine and proline metabolism; glyoxylate and dicarboxylate metabolism; cysteine and methionine metabolism; starch and sucrose metabolism; glycolysis or gluconeogenesis; and pyrimidine metabolism. In addition, elevated [CO] accumulated carbohydrates, 1,2,3-trihydroxybenzene, pyrocatechol, glutamate, and l-gulonolactone, to allow adaption to severe drought. In conclusion, the metabolites and metabolic pathways associated with the alleviation of drought stresses by elevated [CO] were different according to the level of drought stress. Our results may provide a theoretical basis for CO fertilization and application of exogenous metabolites to enhance drought tolerance of cucumber.
大气中二氧化碳浓度升高可在生理水平上缓解黄瓜的中度至重度干旱胁迫。为探究其潜在的代谢机制，对黄瓜幼苗进行了两种二氧化碳浓度和三种水分处理组合的实验，并采用非靶向代谢组学方法对其叶片进行分析。结果表明，在中度干旱胁迫下，二氧化碳浓度升高改变了79种差异代谢物，这些代谢物主要与丙氨酸、天冬氨酸和谷氨酸代谢；精氨酸和脯氨酸代谢；三羧酸循环；以及甘油磷脂代谢有关。此外，二氧化碳浓度升高促进了次生代谢物的积累，包括异阿魏酸、间香豆酸和水杨尿酸。在重度干旱胁迫下，二氧化碳浓度升高改变了26种差异代谢物，主要涉及丙氨酸、天冬氨酸和谷氨酸代谢；丙酮酸代谢；精氨酸和脯氨酸代谢；乙醛酸和二羧酸代谢；半胱氨酸和甲硫氨酸代谢；淀粉和蔗糖代谢；糖酵解或糖异生；以及嘧啶代谢。此外，二氧化碳浓度升高积累了碳水化合物、1,2,3-三羟基苯、邻苯二酚、谷氨酸和L-古洛糖酸内酯，以适应重度干旱。总之，根据干旱胁迫程度的不同，二氧化碳浓度升高缓解干旱胁迫所涉及的代谢物和代谢途径也有所不同。我们的研究结果可为二氧化碳施肥及应用外源代谢物提高黄瓜耐旱性提供理论依据。
