西兰花和花椰菜的硫代葡萄糖苷
Glucosinolates of and .
机构信息
Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.
Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.
出版信息
Molecules. 2022 Dec 2;27(23):8431. doi: 10.3390/molecules27238431.
Glucosinolates (GSLs) from and were analyzed qualitatively and quantitatively by their desulfo-counterparts using UHPLC-DAD-MS/MS. Eight GSLs were identified in , including Val-derived (glucoputranjivin) and Trp-derived (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin) as the major ones followed by Leu-derived (Isobutyl GSL), Ile-derived (glucocochlearin) and Phe/Tyr-derived (glucosinalbin). Different plant parts contained six GSLs, with Met-derived (progoitrin, epiprogoitrin, and gluconapin) and homoPhe-derived (gluconasturtiin) as the major ones, followed by glucosinalbin and neoglucobrassicin. GSL breakdown products obtained by hydrodistillation (HD) and microwave-assisted distillation from , as well as isopropyl isothiocyanate, as the major volatile in both isolates, were tested for their cytotoxic activity using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Generally, all volatile isolates showed similar activity toward the three cancer cell lines. The best activity was shown by isopropyl isothiocyanate at a concentration of 100 µg/mL after 72 h of incubation, with 53.18% for MDA-MB-231, 56.61% for A549, and 60.02% for the T24 cell line.
利用超高效液相色谱-二极管阵列检测-串联质谱联用（UHPLC-DAD-MS/MS），通过其去磺化对应物对 和 中的硫代葡萄糖苷（GSLs）进行定性和定量分析。在 中鉴定出 8 种 GSLs，包括缬氨酸衍生（glucoputranjivin）和色氨酸衍生（4-羟基葡萄糖异硫氰酸酯、葡萄糖异硫氰酸酯、4-甲氧基葡萄糖异硫氰酸酯和新葡萄糖异硫氰酸酯），其次是亮氨酸衍生（异丁基 GSL）、异亮氨酸衍生（glucocochlearin）和苯丙氨酸/酪氨酸衍生（葡萄糖辛纳丁）。不同的 植物部位含有 6 种 GSLs，其中以蛋氨酸衍生（前甘氨酸、表前甘氨酸和葡萄糖辣根素）和同型苯丙氨酸衍生（葡萄糖野黑樱素）为主，其次是葡萄糖辛纳丁和新葡萄糖异硫氰酸酯。通过水蒸馏（HD）和微波辅助蒸馏从 中获得的 GSL 分解产物，以及异丙基异硫氰酸酯，作为两种分离物中的主要挥发性物质，通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物（MTT）测定法测试其细胞毒性活性。通常，所有挥发性分离物对三种癌细胞系均表现出相似的活性。在孵育 72 小时后，浓度为 100 µg/mL 的异丙基异硫氰酸酯表现出最佳活性，对 MDA-MB-231 的抑制率为 53.18%，对 A549 的抑制率为 56.61%，对 T24 细胞系的抑制率为 60.02%。
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