Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.
School of Medicine, University of Split, Šoltanska 2, 21000 Split, Croatia.
Molecules. 2019 Feb 19;24(4):741. doi: 10.3390/molecules24040741.
belongs to the Brassicaceae family, which represents a forgotten crop of the Euro-Mediterranean area. The aim of the present study was to determine the glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, and cytotoxic activities) of the isolates containing glucosinolate breakdown products. The chemical profiles were determined by using HPLC-PDA-MS/MS of desulfoglucosinolates and GC-MS of glucosinolate degradation products. The analysis of showed the presence of seven glucosinolates: gluconapin (), glucoraphasatin (), glucoraphenin (), glucoerucin (), glucoraphanin (), glucotropaeolin (), and glucosinalbin (). The total glucosinolate content ranged from 7.0 to 14.6 µmol/g of dry weight, with the major glucosinolate glucosinalbin in all parts. The antioxidant activity of all volatile isolates was not notable. At a tested concentration of 227 μg/mL, flower hydro-distillate (FH) showed good AChE inhibition, i.e., 40.9%, while root hydro-distillate (RH) had good activity against BChE, i.e., 54.3%. FH showed the best activity against both tested human bladder cancer cell lines, i.e., against T24 after 72 h, which have IC of 16.0 μg/mL, and against TCCSUP after 48 h with IC of 7.8 μg/mL, and can be considered as highly active. On the other hand, RH showed weak activity against tested cancer cells.
属于十字花科，代表了欧洲-地中海地区被遗忘的作物之一。本研究旨在确定不同植物部位的硫代葡萄糖苷（glucosinolate）图谱以及含有硫代葡萄糖苷分解产物的分离物的生物特性（抗氧化、抗胆碱酯酶和细胞毒性活性）。通过使用 HPLC-PDA-MS/MS 测定脱硫硫代葡萄糖苷和 GC-MS 测定硫代葡萄糖苷降解产物来确定化学图谱。分析表明，存在七种硫代葡萄糖苷：葡萄糖苷（gluconapin (））、葡萄糖辣根苷（glucoraphasatin ()）、葡萄糖辣根苷（glucoraphenin ()）、葡萄糖辣根苷（glucoerucin ()）、葡萄糖芸薹素（glucoraphanin ()）、葡萄糖萝卜硫苷（glucotropaeolin ()）和葡萄糖硫苷（glucosinalbin ()）。总硫代葡萄糖苷含量范围为 7.0 至 14.6 µmol/g 干重，所有部位的主要硫代葡萄糖苷均为葡萄糖硫苷。所有挥发性分离物的抗氧化活性都不显著。在 227 μg/mL 的测试浓度下，花水蒸馏物（FH）对 AChE 抑制作用较好，即 40.9%，而根水蒸馏物（RH）对 BChE 活性较好，即 54.3%。FH 对两种测试的人膀胱癌细胞系均表现出良好的活性，即在 72 小时后对 T24 的 IC 为 16.0 μg/mL，在 48 小时后对 TCCSUP 的 IC 为 7.8 μg/mL，可以认为是高度活跃的。另一方面，RH 对测试的癌细胞表现出较弱的活性。
