Liu Chunguang, Han Yujun, Teng Chunhong, Ma Hong, Tao Bo, Yang Fengshan
College of Agriculture, Northeast Agricultural University, Harbin 150030, China; Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
Ecotoxicol Environ Saf. 2023 Jan 1;249:114390. doi: 10.1016/j.ecoenv.2022.114390. Epub 2022 Dec 9.
Florpyrauxifen-benzyl is an herbicide that has been developed in recent years. Its degradation mode in paddy soil environments is not clear. In this study, the degradation dynamics in paddy soil and water were studied by ultrahigh-performance liquid chromatography. Microbial degradation was the main degradation pathway. Using third-generation high-throughput sequencing technology, the changes in the soil bacterial community structure were studied. After 30 days of application, compared with the control group (F0), the abundance of Sphingomonas, Lysobacter, and Flavisolibacter in the recommended and repeated application groups (F1, F5 and F10) increased significantly, and uncultured bacterium and Terrimonas decreased significantly. Compared with the F0 and F1 groups, the species diversity of the F0 and F1 groups showed a significant increase over time. The species diversity of the F5 and F10 groups decreased significantly on Days 5 and 15. On Day 30, the recovery even exceeded that of the control group. Luteimonas and five other genera were positively correlated with herbicide residues, and Pseudolabrys and two other genera were negatively correlated. Repeated application showed a significant effect on the structure of the soil bacterial community, mainly showing a trend of a significant decrease in the initial stage and gradual recovery in the later stage. The results will guide the safe and rational use of florpyrauxifen-benzyl and provide a scientific basis for florpyrauxifen-benzyl dynamic supervision of environmental pollution and protection of black soil in Northeast China.
氟吡草胺苄酯是近年来开发的一种除草剂。其在稻田土壤环境中的降解模式尚不清楚。本研究采用超高效液相色谱法研究了其在稻田土壤和水中的降解动态。微生物降解是主要的降解途径。利用第三代高通量测序技术，研究了土壤细菌群落结构的变化。施药30天后，与对照组（F0）相比，推荐施药组和重复施药组（F1、F5和F10）中鞘氨醇单胞菌属、溶杆菌属和黄杆菌属的丰度显著增加，未培养细菌和地嗜皮菌属显著减少。与F0和F1组相比，F0和F1组的物种多样性随时间显著增加。F5和F10组的物种多样性在第5天和第15天显著下降。在第30天，其恢复程度甚至超过了对照组。藤黄单胞菌属等6个属与除草剂残留呈正相关，假拉布氏菌属等3个属与除草剂残留呈负相关。重复施药对土壤细菌群落结构有显著影响，主要表现为初期显著下降、后期逐渐恢复的趋势。研究结果将指导氟吡草胺苄酯的安全合理使用，为东北地区氟吡草胺苄酯环境污染动态监管及黑土保护提供科学依据。
