Gao Zihao, Zheng Yu, Li Zhendong, Ruan Aidong
The National Key Laboratory of Water Disaster Prevention, Hohai University, Nanjing 210098, China.
College of Hydrology and Water Resources, Hohai University, Nanjing 210098, China.
Toxics. 2024 May 19;12(5):373. doi: 10.3390/toxics12050373.
17β-Estradiol (E2) is a widely present trace pollutant in aquatic environments. However, its impact on microbial communities in aerobic lake waters, which are crucial for methane (CH) production, remains unclear. This study conducted an E2 contamination experiment by constructing laboratory-simulated aerobic microecosystems. Using 16S rRNA high-throughput sequencing, the effects of E2 on bacterial and archaeal communities were systematically examined. Combined with gas chromatography, the patterns and mechanisms of E2's impact on CH emissions in aerobic aquatic systems were uncovered for the first time. Generally, E2 contamination increased the randomness of bacterial and archaeal community assemblies and weakened microbial interactions. Furthermore, changes occurred in the composition and ecological functions of bacterial and archaeal communities under E2 pollution. Specifically, two days after exposure to E2, the relative abundance of in the low-concentration (L) and high-concentration (H) groups decreased by 6.99% and 4.01%, respectively, compared to the control group (C). Conversely, the relative abundance of was 1.81% and 1.60% higher in the L and H groups, respectively. E2 contamination led to an increase in the relative abundance of the functional group and a decrease in that of the functional group. These changes led to an increase in CH emissions. This study comprehensively investigated the ecotoxicological effects of E2 pollution on microbial communities in aerobic water bodies and filled the knowledge gap regarding aerobic methane production under E2 contamination.
17β-雌二醇（E2）是水生环境中广泛存在的痕量污染物。然而，其对需氧湖水中微生物群落的影响尚不清楚，而需氧湖水对甲烷（CH）的产生至关重要。本研究通过构建实验室模拟需氧微生态系统进行了E2污染实验。利用16S rRNA高通量测序技术，系统研究了E2对细菌和古菌群落的影响。结合气相色谱法，首次揭示了E2对需氧水生系统中CH排放的影响模式及机制。总体而言，E2污染增加了细菌和古菌群落组装的随机性，削弱了微生物间的相互作用。此外，E2污染下细菌和古菌群落的组成及生态功能发生了变化。具体而言，暴露于E2两天后，低浓度（L）组和高浓度（H）组中 的相对丰度与对照组（C）相比分别下降了6.99%和4.01%。相反，L组和H组中 的相对丰度分别高出1.81%和1.60%。E2污染导致 功能组的相对丰度增加， 功能组的相对丰度降低。这些变化导致CH排放量增加。本研究全面调查了E2污染对需氧水体中微生物群落的生态毒理学效应，填补了E2污染下需氧甲烷产生方面的知识空白。
