尼日利亚西南部豇豆病毒研究进展：分布、流行情况及混合感染

Updates on cowpea viruses in Southwest Nigeria: distribution, prevalence and coinfection.

作者信息

Ogunsola Kayode Ezekiel, Yusuf Abubakar, Elegbeku Olusegun Akinleye

机构信息

Department of Biological Sciences, (Biotechnology Programme), Bells University of Technology, PMB 1015, Ota, Ogun State Nigeria.

Department of Biological Sciences, Federal University Dutsinma, PMB 5001, Dutsinma, Katsina State Nigeria.

出版信息

Indian Phytopathol. 2023;76(1):201-213. doi: 10.1007/s42360-022-00576-8. Epub 2022 Dec 4.

DOI:10.1007/s42360-022-00576-8

PMID:36531908

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9734776/

Abstract

UNLABELLED

Cowpea is an important source of dietary proteins in the semi-arid regions of sub-Saharan Africa. Its productivity is constrained by several viral diseases and there are limited updates on the incidence and distribution of these diseases in Nigeria. This study assessed the distribution and prevalence of cowpea viruses in Southwest Nigeria. Field surveys were conducted in 2017 and 2018, in which a total of 600 leaf samples were randomly collected from 60 cowpea fields in four (Oyo, Ogun, Ondo and Osun) states at 15 fields per state and 10 samples per field. Disease incidence and severity were recorded while virus infections were confirmed by enzyme-linked immunosorbent assay or reverse transcription polymerase chain reaction. Viral disease symptoms of systemic mosaic, mottling, puckering, vein-banding, leaf deformation and stunted growth were observed. Highest virus incidence and severity (100% and 4.8 ± 0.4) were observed at Adeosun Avenue, Ondo state, whereas Boredun, Osun state had the least (80% and 3.8 ± 0.7), with some symptomless fields found among the states. Seven viruses, viz.: cowpea aphid-borne mosaic virus (CABMV), cowpea mild mottle virus (CPMMV), bean common mosaic virus-blackeye cowpea mosaic strain (BCMV-BlCM), cucumber mosaic virus (CMV), southern bean mosaic virus (SBMV), cowpea mottle virus (CMoV) and cowpea yellow mosaic virus (CYMV) were detected from 173 (28.8%) samples collected from 32 (53.3%) fields across the states. CPMMV was prevalent, detected from 30.0% of surveyed fields, whereas CYMV was the least prevalent (3.3%). Multiple infections of two to four viruses were observed among 12.5% of samples from 51.7% of fields. Highest incidence of single and multiple virus infections were observed in Ondo state. This updates on virus distributions in Southwest Nigeria will be useful for multiple virus resistance-breeding programs and other viral disease management strategies for improved cowpea productivity.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s42360-022-00576-8.

摘要

未标注

豇豆是撒哈拉以南非洲半干旱地区膳食蛋白质的重要来源。其生产力受到多种病毒病的制约，而关于尼日利亚这些病害的发病率和分布情况的最新信息有限。本研究评估了尼日利亚西南部豇豆病毒的分布和流行情况。在2017年和2018年进行了田间调查，共从四个州（奥约州、奥贡州、翁多州和奥孙州）的60个豇豆田随机采集了600片叶片样本，每个州15个田块，每个田块10个样本。记录了病害的发病率和严重程度，同时通过酶联免疫吸附测定或逆转录聚合酶链反应确认病毒感染情况。观察到系统性花叶、斑驳、起皱、叶脉带、叶片变形和生长受阻等病毒病症状。在翁多州的阿德奥孙大道观察到最高的病毒发病率和严重程度（100%和4.8±0.4），而奥孙州的博雷敦最低（80%和3.8±0.7），各州市中也发现了一些无症状田块。从各州32个（53.3%）田块采集的173个（28.8%）样本中检测到七种病毒，即：豇豆蚜传花叶病毒（CABMV）、豇豆轻斑驳病毒（CPMMV）、菜豆普通花叶病毒-黑眼豇豆花叶株系（BCMV-BlCM）、黄瓜花叶病毒（CMV）、南方菜豆花叶病毒（SBMV）、豇豆斑驳病毒（CMoV）和豇豆黄花叶病毒（CYMV）。CPMMV普遍存在，在30.0%的调查田块中被检测到，而CYMV的流行率最低（3.3%）。在51.7%的田块中，12.5%的样本观察到两种至四种病毒的多重感染。翁多州观察到单一和多重病毒感染的最高发病率。尼日利亚西南部病毒分布的这一最新情况将有助于开展多重病毒抗性育种计划以及其他病毒病管理策略，以提高豇豆的生产力。