College of Agronomy, Tarim University, Alar 843300, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Genes (Basel). 2022 Nov 23;13(12):2194. doi: 10.3390/genes13122194.
(), Leucine-rich repeats in F-box proteins, belongs to the Skp1-Cullin1-F-box protein (SCF) E3 ligase family. genes play important roles in plant growth, such as plant hormones, responses to environmental stress, and floral organ development. Here, a total of 518 genes were identified and analyzed in six plant species. Phylogenetic analysis showed that s, s, and s were clustered into three subfamilies (Ⅰ-Ⅲ). Based on the composition of the F-box domain and carboxyl-terminal amino acid sequence, FBXL proteins were classified into three types (Type-A/-B/-C). Whole-genome duplication (WGD) along with tandem duplications and segmental contributed to the expansion of this gene family. The result indicates that four cotton species are also divided into three subfamilies. s in cotton were classified into three clades by phylogenetic and structural analyses. Furthermore, expression analyses indicated that the expression patterns of s in different cotton tissues were different. The highly expressed of in 5-8 mm anthers, indicates that this gene might play a role in the reproductive process, providing candidate genes for future studies on cotton fertility materials. This study provides an original functional opinion and a useful interpretation of the FBXL protein family in cotton.
富含亮氨酸重复的 F-box 蛋白属于 Skp1-Cullin1-F-box 蛋白（SCF）E3 连接酶家族。这些基因在植物生长中发挥着重要作用，如植物激素、环境胁迫响应和花器官发育。在这里，我们在六种植物物种中鉴定和分析了总共 518 个基因。系统发育分析表明，s、s 和 s 被聚类为三个亚家族（Ⅰ-Ⅲ）。基于 F-box 结构域和羧基末端氨基酸序列，FBXL 蛋白被分为三种类型（Type-A/-B/-C）。全基因组复制（WGD）以及串联重复和片段化导致了这个基因家族的扩张。结果表明，四个棉花物种也分为三个亚家族。棉铃中的 s 通过系统发育和结构分析被分为三个分支。此外，表达分析表明，s 在不同棉花组织中的表达模式不同。在 5-8mm 花药中高度表达的 表明该基因可能在生殖过程中发挥作用，为未来研究棉花育性材料提供了候选基因。本研究为 FBXL 蛋白家族在棉花中的功能提供了一个新的观点和有用的解释。
