Berkowitz R S, Hill J A, Kurtz C B, Anderson D J
Fearing Laboratory, Brigham and Women's Hospital, Boston, MA 02115.
Am J Obstet Gynecol. 1988 Jan;158(1):199-203. doi: 10.1016/0002-9378(88)90810-1.
Supernatants from activated leukocyte cultures and individual lymphokines and monokines were added to cultures of JEG-3 human gestational choriocarcinoma cells in vitro, and effects on cell proliferation were measured. Activated leukocyte culture supernatants, recombinant gamma-interferon, tumor necrosis factor, and colony-stimulating factor significantly inhibited JEG-3 proliferation. In contrast, high doses of both interleukin 1 and 2 stimulated JEG-3 proliferation. Low doses of B cell growth factor stimulated JEG-3 proliferation, whereas the highest dose was inhibitory. Further understanding of the effects of lymphokines and monokines on trophoblastic growth may provide important insights into immunologic mechanisms affecting early pregnancy development and tumor-host interactions in gestational trophoblastic neoplasia.
将活化白细胞培养物的上清液以及单个淋巴因子和单核因子添加到体外培养的JEG-3人妊娠绒毛膜癌细胞中，并检测其对细胞增殖的影响。活化白细胞培养物上清液、重组γ干扰素、肿瘤坏死因子和集落刺激因子显著抑制JEG-3细胞增殖。相比之下，高剂量的白细胞介素1和2刺激JEG-3细胞增殖。低剂量的B细胞生长因子刺激JEG-3细胞增殖，而高剂量则具有抑制作用。进一步了解淋巴因子和单核因子对滋养层生长的影响，可能为影响早期妊娠发育的免疫机制以及妊娠滋养细胞肿瘤中的肿瘤-宿主相互作用提供重要见解。
