Evaluation of Phosphine Resistance in Populations of , and in the Czech Republic.

Phosphine is globally the most widely adopted fumigant for the control of storage pests. Recently, an increase in the frequency of stored-product pest resistance has been observed with significant geographical and interspecific variations. In this context, there are available data for the occurrence of resistant populations from America, Asia, Africa, and Australia, but there are few data in the case of Europe. Therefore, the aim of this work was to evaluate phosphine efficacy in important beetle pests of stored products, i.e., (L.), (L.), and (F.) sampled from the Czech Republic, using a rapid diagnostic test that is based on the speed to knockdown after exposure. Apart from the standard laboratory populations, which were used as the controls, we tested 56 field populations of these three species, collected in Czech farm grain stores. The survey revealed that 57.1% of the tested field populations were classified as phosphine-susceptible, based on the knockdown method used. However, profound variations among species and populations were recorded. The species with the highest percentage of resistant populations was (71.4% of the populations; resistance coefficient 0.5-4.1), followed by (57.1% of the populations; resistance coefficient 0.8-6.9), and (9.5% of the populations; resistance coefficient 0.5-2.9). Regarding the intra-population variability in response to phosphine (slope of the knockdown time regression), the laboratory and slightly resistant populations of all species were homogenous, whereas the most resistant populations were strongly heterogeneous. Our data show that the occurrence of resistance in the Czech Republic is relatively widespread and covers a wide range of species, necessitating the need for the adoption of an action plan for resistance mitigation.