College of Pharmacy, Jinan University, Guangzhou 511443, China.
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China.
Int J Mol Sci. 2022 Dec 13;23(24):15850. doi: 10.3390/ijms232415850.
As one of the most studied mesoporous silica nanoparticles (MSNs) in drug delivery systems, Mobil Composition of Matter No. 41 (MCM-41) possesses unique properties including perfect channel architecture, excellent load capacity, and good biocompatibility. However, the applications of MCM-41 nanoparticles in drug delivery have not yet been industrialized, due to the interaction between MCM-41 and biomolecules (especially proteins) that affect their in vivo behaviors after dosing. To investigate the interactions between MCM-41 and proteins, this study selected bovine serum albumin (BSA), lysozyme (Lyso), and bovine hemoglobin (BHb) as model proteins and characterized the ultraviolet-visible, fluorescence, circular dichroism spectra and the protein adsorption of MCM-41-protein complex. The UV-Vis spectra exhibited the different absorption increment degrees of three proteins. The fluorescence spectra showed that the fluorescence intensity of proteins changed by different trends. The CD spectra indicated that the secondary structure changes were ranked as BSA > Lyso > BHb, which is consistent with the protein’s adsorption capability on MCM-41. It was shown that there were three different patterns of MCM-41-proteins interactions. The hydrophilic and low-charged BSA followed the strong interaction pattern, the hydrophilic but heavily charged Lyso followed the moderate interaction pattern, and the hydrophobic BHb followed the weak interaction pattern. Different interaction patterns would lead to different effects on the structural properties of proteins, the surface chemistry of MCM-41, and the absorption capability of proteins on MCM-41. We believe our study will provide a better insight into the application of MCM-41 nanoparticles in drug delivery systems.
作为药物输送系统中研究最多的介孔硅纳米颗粒（MSNs）之一，Mobil Composition of Matter No. 41（MCM-41）具有独特的性质，包括完美的通道结构、优异的负载能力和良好的生物相容性。然而，由于 MCM-41 与生物分子（特别是蛋白质）之间的相互作用，影响了它们给药后的体内行为，因此 MCM-41 纳米颗粒在药物输送中的应用尚未实现工业化。为了研究 MCM-41 与蛋白质之间的相互作用，本研究选择牛血清白蛋白（BSA）、溶菌酶（Lyso）和牛血红蛋白（BHb）作为模型蛋白，并对 MCM-41-蛋白复合物的紫外可见、荧光、圆二色光谱和蛋白吸附进行了表征。紫外可见光谱显示了三种蛋白质不同的吸光度增加程度。荧光光谱表明，蛋白质的荧光强度发生了不同的变化趋势。圆二色光谱表明，二级结构变化的顺序为 BSA > Lyso > BHb，与蛋白质在 MCM-41 上的吸附能力一致。结果表明，MCM-41-蛋白质相互作用存在三种不同模式。亲水且低电荷的 BSA 遵循强相互作用模式，亲水但带高电荷的 Lyso 遵循中等相互作用模式，疏水性 BHb 遵循弱相互作用模式。不同的相互作用模式会对蛋白质的结构特性、MCM-41 的表面化学性质以及蛋白质在 MCM-41 上的吸附能力产生不同的影响。我们相信，我们的研究将为更好地了解 MCM-41 纳米颗粒在药物输送系统中的应用提供深入的认识。
