Curl Casey C, Kumar Anika, Peck Austin J, Arevalo Jose A, Gleason Allison, Leija Robert G, Osmond Adam D, Duong Justin J, Miller Benjamin F, Horning Michael A, Brooks George A
Department of Integrative Biology, University of California at Berkeley, Berkeley, CA 94720, USA.
Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
Metabolites. 2022 Nov 23;12(12):1163. doi: 10.3390/metabo12121163.
Patients treated for traumatic brain injury (TBI) are in metabolic crises because of the trauma and underfeeding. We utilized fractional gluconeogenesis (fGNG) to assess nutritional adequacy in ad libitum-fed and calorically-restricted rats following TBI. Male Sprague-Dawley individually housed rats 49 days of age were randomly assigned into four groups: ad libitum (AL) fed control (AL-Con, sham), AL plus TBI (AL+TBI), caloric restriction (CR) control (CR-Con, sham), and CR plus TBI (CR+TBI). From days 1-7 animals were given AL access to food and water containing 6% deuterium oxide (DO). On day 8, a pre-intervention blood sample was drawn from each animal, and TBI, sham injury, and CR protocols were initiated. On day 22, the animals were euthanized, and blood was collected to measure fGNG. Pre-intervention, there was no significant difference in fGNG among groups ( ≥ 0.05). There was a significant increase in fGNG due to caloric restriction, independent of TBI ( ≤ 0.05). In addition, fGNG may provide a real-time, personalized biomarker for assessing patient dietary caloric needs.
因创伤和营养不足，创伤性脑损伤（TBI）患者处于代谢危机之中。我们利用分数糖异生（fGNG）来评估TBI后自由进食和热量限制大鼠的营养充足情况。将49日龄单独饲养的雄性Sprague-Dawley大鼠随机分为四组：自由进食（AL）对照组（AL-Con，假手术）、AL加TBI组（AL+TBI）、热量限制（CR）对照组（CR-Con，假手术）和CR加TBI组（CR+TBI）。从第1天到第7天，让动物自由获取含有6%氧化氘（DO）的食物和水。在第8天，从每只动物身上采集干预前血样，并启动TBI、假手术损伤和CR方案。在第22天，对动物实施安乐死，并采集血液以测量fGNG。干预前，各组间fGNG无显著差异（≥0.05）。热量限制导致fGNG显著增加，与TBI无关（≤0.05）。此外，fGNG可能为评估患者饮食热量需求提供一种实时、个性化的生物标志物。
