Teoh Chin May, Cooper Analynn, Renteria Karisa M, Lane Michelle, Zhu Jie, Koh Gar Yee
Nutrition and Foods Program, School of Family and Consumer Sciences, Texas State University, San Marcos, TX 78666, USA.
Metabolites. 2022 Dec 12;12(12):1252. doi: 10.3390/metabo12121252.
A Western-style diet that is high in fat and sucrose has been shown to alter DNA methylation and epigenetically modify genes related to health risk in offspring. Here, we investigated the effect of a methyl-donor nutrient (MS) supplemented to a high-fat, high-sucrose (HFS) diet during pregnancy and lactation on vitamin D (VD) status and inflammatory response in offspring. After mating, 10-week-old female Sprague-Dawley (SD) rats ( = 10/group) were randomly assigned to one of the four dietary groups during pregnancy and lactation: (1) control diet (CON), (2) CON with MS (CON-MS), (3) HFS, and (4) HFS with MS (HFS-MS). Weanling offspring (three weeks old) were euthanized and sacrificed ( = 8-10/sex/group). The remaining offspring ( = 10/sex/group) were randomly assigned to either a CON or an HFS diet for 12 weeks and sacrificed at 15 weeks of age. Our results indicated that prenatal MS supplementation, but not postnatal diet, restored low vitamin D status and suppressed elevation of proinflammatory cytokine induced by maternal HFS in the offspring. Furthermore, both prenatal and postnatal diets modulated the abundance of spp. and spp. in the offspring, a shift that was independent of vitamin D status. Collectively, our data support a role for MS in restoring the perturbation of VD status and normalizing maternal HFS-induced inflammation in the offspring. Further investigation is warranted to elucidate the methylation status of VD metabolism-related pathways in the offspring, as well as the immunomodulatory role of vitamin D during the progression of obesity.
高脂肪和高蔗糖的西式饮食已被证明会改变DNA甲基化，并在表观遗传上修饰与后代健康风险相关的基因。在此，我们研究了在怀孕和哺乳期向高脂肪、高蔗糖（HFS）饮食中添加甲基供体营养素（MS）对后代维生素D（VD）状态和炎症反应的影响。交配后，将10周龄的雌性Sprague-Dawley（SD）大鼠（每组n = 10）在怀孕和哺乳期随机分配到四个饮食组之一：（1）对照饮食（CON），（2）添加MS的CON（CON-MS），（3）HFS，以及（4）添加MS的HFS（HFS-MS）。对断奶后代（三周龄）实施安乐死并进行处死（每组雌雄各8 - 10只）。将其余后代（每组雌雄各10只）随机分配到CON或HFS饮食中，持续12周，并在15周龄时处死。我们的结果表明，产前补充MS可恢复后代低维生素D状态，并抑制母体HFS诱导的后代促炎细胞因子升高，而后天饮食则无此作用。此外，产前和产后饮食均调节了后代中特定菌种的丰度，这种变化与维生素D状态无关。总体而言，我们的数据支持MS在恢复后代VD状态紊乱和使母体HFS诱导的炎症正常化方面的作用。有必要进一步研究以阐明后代中VD代谢相关途径的甲基化状态，以及肥胖进展过程中维生素D的免疫调节作用。
