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Identification and classification of Campylobacter strains by using nonradioactive DNA probes.

作者信息

Chevrier D, Larzul D, Megraud F, Guesdon J L

机构信息

Laboratoire des Sondes Froidés, Institut Pasteur, Paris, France.

出版信息

J Clin Microbiol. 1989 Feb;27(2):321-6. doi: 10.1128/jcm.27.2.321-326.1989.

DOI:10.1128/jcm.27.2.321-326.1989
PMID:2915027
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267300/
Abstract

Acetylaminofluorene-labeled genomic DNA probes were used for the identification and classification of Campylobacter strains. Relationships among 17 well-known strains of Campylobacter species and subspecies were studied by comparing acetylaminofluorene- or 32P-labeled probes. Results obtained with both methods were closely correlated and were in agreement with those already reported. In an identification experiment, hybridization with nonradioactive probes was performed on 60 strains isolated from stool samples after subculturing and quick DNA extraction; conventional biochemical tests were conducted in parallel. A good correlation was found between the results obtained by nonradioactive hybridization and by biochemical tests. Thus, the acetylaminofluorene-labeled genomic DNA probe method is an interesting alternative for laboratories without access to radioisotopes for the identification and classification of bacteria.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bbd/267300/3e98d57e5a93/jcm00062-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bbd/267300/61432fdb1656/jcm00062-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bbd/267300/3e98d57e5a93/jcm00062-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bbd/267300/61432fdb1656/jcm00062-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bbd/267300/3e98d57e5a93/jcm00062-0109-a.jpg

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本文引用的文献

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Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.通过DNA菌落杂交法检测产肠毒素大肠杆菌
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DNA relatedness among strains of Campylobacter jejuni and Campylobacter coli with divergent serogroup and hippurate reactions.空肠弯曲菌和结肠弯曲菌菌株之间的DNA相关性，这些菌株具有不同的血清群和马尿酸盐反应。
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A comparative study of clinical and food isolates of Listeria monocytogenes and related species.单核细胞增生李斯特菌及相关菌种临床分离株与食品分离株的比较研究
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Isolation of a urease positive thermophilic variant of Campylobacter lari from a patient with urinary tract infection.从一名尿路感染患者中分离出一株脲酶阳性的嗜热弯曲菌变体。
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DNA probes for diagnosis of intestinal infection.用于诊断肠道感染的DNA探针。
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Oligodeoxynucleotide probes for Campylobacter fetus and Campylobacter hyointestinalis based on 16S rRNA sequences.基于16S rRNA序列的胎儿弯曲杆菌和空肠弯曲杆菌的寡脱氧核苷酸探针。
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10
Occurrence of aminoglycoside phosphotransferase subclass I and II structural genes among Enterobacteriaceae spp. isolated from meat samples.从肉类样本中分离出的肠杆菌科细菌中氨基糖苷磷酸转移酶I类和II类结构基因的出现情况。
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4
Nitrocellulose filter blots for species identification of Mobiluncus curtisii and Mobiluncus mulieris.用于柯氏动弯杆菌和女性动弯杆菌菌种鉴定的硝酸纤维素滤膜印迹法。
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2-Acetylaminofluorene-modified probes for the indirect hybridocytochemical detection of specific nucleic acid sequences.用于间接杂交细胞化学检测特定核酸序列的2-乙酰氨基芴修饰探针。
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Immunochemistry. 1969 Jan;6(1):43-52. doi: 10.1016/0019-2791(69)90177-3.