Mathlouthi Nour El Houda, Kriaa Aicha, Keskes Leila Ammar, Rhimi Moez, Gdoura Radhouane
Laboratoire de Recherche Toxicologie Microbiologie Environnementale et Santé (LR17ES06), Faculté des Sciences de Sfax, Université de Sfax, Sfax 3000, Tunisia.
Microbiota Interaction with Human and Animal Team (MIHA), Micalis Institute-UMR1319, Ag-roParisTech, Université Paris-Saclay, INRAE, F-78350 Jouy-en-Josas, France.
Microorganisms. 2022 Nov 30;10(12):2365. doi: 10.3390/microorganisms10122365.
Colorectal cancer (CRC) is a growing public health challenge, featuring a multifactorial etiology and complex host-environment interactions. Recently, increasing evidence has pointed to the role of the gut microbiota in CRC development and progression. To explore the role of gut microbes in CRC, we retrieved metagenomic data from 156 stools from the European Nucleotide Archive database and mapped them against the VFDB database for virulence factors (VFs). GO annotations of VFs and KEGG pathways were then performed to predict the microbial functions and define functional pathways enriched in the tumor-associated microbiota. Interestingly, 306 VFs were detected in the metagenomic data. We revealed the enrichment of adenomas with VFs involved in cell adhesion, whereas in the early stages of CRC they were enriched in both adhesins and isochorismatase. Advanced stages of CRC were enriched with microbial siderophores, especially enterobactin, which was significantly associated with isochorismate synthase. We highlighted higher abundances of porins and transporters involved in antibiotic resistance and the development of biofilm in advanced stages of CRC. Most VFs detected in CRC, particularly in advanced stages, were shown to be included in siderophore biosynthesis pathways. This enrichment of predicted VFs supports the key role of the gut microbiota in the disease.
结直肠癌（CRC）是一个日益严峻的公共卫生挑战，其病因多因素且宿主 - 环境相互作用复杂。最近，越来越多的证据表明肠道微生物群在CRC的发生和发展中起作用。为了探究肠道微生物在CRC中的作用，我们从欧洲核苷酸档案数据库中检索了156份粪便的宏基因组数据，并将它们与毒力因子（VFs）的VFDB数据库进行比对。然后对VFs进行基因本体（GO）注释和京都基因与基因组百科全书（KEGG）通路分析，以预测微生物功能并确定肿瘤相关微生物群中富集的功能通路。有趣的是，在宏基因组数据中检测到306种VFs。我们发现腺瘤中富含参与细胞黏附的VFs，而在CRC早期，它们在黏附素和异分支酸酶中均有富集。CRC晚期富含微生物铁载体，尤其是肠杆菌素，其与异分支酸合酶显著相关。我们强调在CRC晚期，参与抗生素抗性和生物膜形成的孔蛋白和转运蛋白丰度更高。在CRC中检测到的大多数VFs，特别是在晚期，都显示包含在铁载体生物合成途径中。预测的VFs的这种富集支持了肠道微生物群在该疾病中的关键作用。
