Department of Medical Biochemistry, University of Ghana Medical School, Korle-Bu, Accra, Ghana.
School of Infection & Immunity, University of Glasgow, Glasgow, UK.
Sci Rep. 2024 Oct 5;14(1):23195. doi: 10.1038/s41598-024-74299-3.
Colorectal cancer (CRC) is a severe gastrointestinal cancer and a leading cause of cancer-related deaths in Ghana. The potential role of gut Enterobacteriaceae in the increasing incidence of CRC in Ghana is yet to be thoroughly investigated. In this study, Enterobacteriaceae from CRC patients and healthy control participants were analyzed by whole genome sequencing to identify genomic features that are associated with CRC. Socio-demographic data showed a significant association between age and alcohol consumption and CRC. Escherichia coli was the most abundant Enterobacteriaceae isolated from the study participants and they were predominantly intestinal commensals. Escherichia coli isolates belonging to phylogroup D encoded the highest number of virulence genes. The agn43 and int genes were widespread in Escherichia coli isolates from the CRC patients. Multilocus sequence types of potentially pathogenic Escherichia coli from the CRC patients also encoded genes involved in aggregation, adherence and biofilm formation. The ampC2 and ampH antimicrobial resistance genes were also widespread in the genome of the Escherichia coli isolates. This study highlights the virulence tendencies of Escherichia coli from CRC patients and their ability to transfer virulence determinants to other Enterobacteriaceae residing in the gut.
结直肠癌（CRC）是一种严重的胃肠道癌症，也是加纳癌症相关死亡的主要原因。肠道肠杆菌科在加纳 CRC 发病率上升中的潜在作用尚未得到彻底研究。在这项研究中，通过全基因组测序分析了 CRC 患者和健康对照参与者的肠杆菌科，以确定与 CRC 相关的基因组特征。社会人口统计学数据显示，年龄和饮酒与 CRC 之间存在显著关联。从研究参与者中分离出的大肠杆菌是最丰富的肠杆菌科，它们主要是肠道共生菌。属于肠杆菌科 D 群的大肠杆菌分离株编码了数量最多的毒力基因。在 CRC 患者的大肠杆菌分离株中，agn43 和 int 基因广泛存在。来自 CRC 患者的潜在致病性大肠杆菌的多位点序列类型也编码了聚集、粘附和生物膜形成相关的基因。ampC2 和 ampH 抗菌药物耐药基因也在大肠杆菌分离株的基因组中广泛存在。本研究强调了来自 CRC 患者的大肠杆菌的毒力倾向及其将毒力决定因素转移到肠道中其他肠杆菌科的能力。
