在一项针对局限性侵袭性牙周炎的纵向研究中，放线共生放线杆菌、中间普氏菌和福赛拟杆菌的联合体存在于骨丢失之前的部位。
A consortium of Aggregatibacter actinomycetemcomitans, Streptococcus parasanguinis, and Filifactor alocis is present in sites prior to bone loss in a longitudinal study of localized aggressive periodontitis.
机构信息
Department of Oral Biology, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA.
出版信息
J Clin Microbiol. 2013 Sep;51(9):2850-61. doi: 10.1128/JCM.00729-13. Epub 2013 Jun 19.
Aggregatibacter actinomycetemcomitans-induced localized aggressive periodontitis (LAP) in African-American adolescents has been documented but is poorly understood. Two thousand fifty-eight adolescents aged 11 to 17 years were screened for their periodontal status and the presence of A. actinomycetemcomitans in their oral cavity. Seventy-one A. actinomycetemcomitans-negative and 63 A. actinomycetemcomitans-positive periodontally healthy subjects were enrolled, sampled, examined, and radiographed yearly for 3 years. Gingival and periodontal pocket depth and attachment levels were recorded. Disease presentation was characterized by bone loss (BL). Subgingival sites were sampled every 6 months to assess (i) the role of A. actinomycetemcomitans in BL and (ii) the association of A. actinomycetemcomitans and other microbes in their relationships to BL. Sixteen of 63 subjects with A. actinomycetemcomitans developed BL (the other 47 subjects with A. actinomycetemcomitans had no BL). No A. actinomycetemcomitans-negative subjects developed BL. Human oral microbe identification microarray (HOMIM) was used for subgingival microbial assessment. On a subject level, pooled data from A. actinomycetemcomitans-positive subjects who remained healthy had higher prevalences of Streptococcus and Actinomyces species, while A. actinomycetemcomitans-positive subjects with BL had higher prevalences of Parvimonas micra, Filifactor alocis, A. actinomycetemcomitans, and Peptostreptococcus sp. human oral taxon 113 (HOT-113). At vulnerable sites, A. actinomycetemcomitans, Streptococcus parasanguinis, and F. alocis levels were elevated prior to BL. In cases where the three-organism consortium (versus A. actinomycetemcomitans alone) was detected, the specificity for detecting sites of future BL increased from 62% to 99%, with a sensitivity of 89%. We conclude that detecting the presence of A. actinomycetemcomitans, S. parasanguinis, and F. alocis together indicates sites of future BL in LAP. A synergistic interaction of this consortium in LAP causation is possible and is the subject of ongoing research.
已记录到伴放线放线杆菌（Aggregatibacter actinomycetemcomitans）引起的非裔美国青少年局限性侵袭性牙周炎（Localized aggressive periodontitis，LAP），但目前对此了解甚少。对 2058 名年龄在 11 至 17 岁的青少年进行牙周状况筛查，并检测其口腔中是否存在伴放线放线杆菌。选取 71 名伴放线放线杆菌阴性和 63 名伴放线放线杆菌阳性的牙周健康受试者，每年对其进行一次口腔检查、采样、检查和拍摄 X 光片，持续 3 年。记录牙龈和牙周袋深度以及附着水平。疾病表现为骨丧失（Bone loss，BL）。每 6 个月对龈下部位进行采样，以评估（i）伴放线放线杆菌在 BL 中的作用，（ii）伴放线放线杆菌与其他微生物的关联及其与 BL 的关系。在 63 名伴放线放线杆菌阳性的受试者中，有 16 名出现 BL（其余 47 名伴放线放线杆菌阳性的受试者没有 BL）。在伴放线放线杆菌阴性的受试者中没有出现 BL。采用人类口腔微生物鉴定微阵列（Human oral microbe identification microarray，HOMIM）对龈下微生物进行评估。在伴放线放线杆菌阳性的健康受试者中，汇总数据显示，普雷沃氏菌属和放线菌属的流行率较高，而 BL 阳性的伴放线放线杆菌阳性受试者中，微小消化链球菌、福赛斯坦纳菌和伴放线放线杆菌以及人类口腔分类单元 113（Peptostreptococcus sp. human oral taxon 113，HOT-113）的流行率较高。在易感部位，BL 发生前，伴放线放线杆菌、中间普雷沃菌和福赛斯坦纳菌的水平升高。在检测到三联菌（与单独的伴放线放线杆菌相比）的情况下，检测未来 BL 部位的特异性从 62%增加到 99%，敏感性为 89%。我们得出结论，检测到伴放线放线杆菌、中间普雷沃菌和福赛斯坦纳菌的存在表明 LAP 中有未来的 BL 部位。这种三联体在 LAP 发病机制中的协同作用是可能的，也是正在进行的研究的主题。
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