Ramiharimanana Fenia D, Haddad Juliano G, Andrianavalonirina Maminiaina A, Apel Cécile, Olivon Florent, Diotel Nicolas, Desprès Philippe, Ramanandraibe Voahangy Vestalys, El Kalamouni Chaker
Unité Mixte Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, INSERM U1187, CNRS UMR 9192, IRD UMR 249, Université de la Réunion, 94791 Sainte Clotilde, France.
International Associated Laboratory, University of Antananarivo-Lyon 1, Antananarivo P.O. Box 906, Madagascar.
Pharmaceuticals (Basel). 2022 Nov 30;15(12):1500. doi: 10.3390/ph15121500.
Dengue and Zika viruses are identified as the most medically important arthropod-borne viral pathogens. Over the past 20 years, the global dengue incidence has dramatically increased with epidemics of severe dengue where the case fatality rate can reach up to 20% in untreated patients. The association between Zika virus infection and severe congenital anomalies was first reported in 2015. Today no specific antiviral therapies are available for dengue and Zika virus infections, accentuating the need of adapted antiviral strategies based on medicinal plant drug discovery. Plants are a potential source of antiviral phytocompounds which act primarily by blocking virus entry in the host-cell. In the present study, we evaluated whether crude extracts from DC. and DC., two endemic plants from Madagascar, may have antiviral effects against dengue and Zika viruses. We showed that has virucidal action whereas inhibits the early steps of virus infection with a non-cytotoxic effect in human cells. The administration of and extracts in zebrafish had no effect on the behavior of animals at the active doses against dengue and Zika viruses, suggesting the absence of adverse effects at these doses. LC-HRMS and molecular networking analyses revealed the richness of these two plants in polyphenols and flavonoid with the presence of clusters of phytocompounds specific to each species. Consequently, and represent potential sources for natural and safe antiviral phytocompounds against flaviviruses of medical concern.
登革热病毒和寨卡病毒被认为是医学上最重要的节肢动物传播病毒病原体。在过去20年里，全球登革热发病率急剧上升，严重登革热疫情频发，未经治疗的患者病死率可达20%。寨卡病毒感染与严重先天性异常之间的关联于2015年首次报道。如今，尚无针对登革热病毒和寨卡病毒感染的特效抗病毒疗法，这凸显了基于药用植物药物研发的适应性抗病毒策略的必要性。植物是抗病毒植物化合物的潜在来源，其主要作用方式是阻断病毒进入宿主细胞。在本研究中，我们评估了来自马达加斯加的两种本土植物——[具体植物1]和[具体植物2]的粗提物是否对登革热病毒和寨卡病毒具有抗病毒作用。我们发现[具体植物1]具有杀病毒作用，而[具体植物2]在人细胞中可抑制病毒感染的早期步骤且无细胞毒性作用。在斑马鱼中给予[具体植物1]和[具体植物2]提取物，在针对登革热病毒和寨卡病毒的有效剂量下对动物行为无影响，表明这些剂量下无不良影响。液相色谱 - 高分辨质谱（LC - HRMS）和分子网络分析显示，这两种植物富含多酚和黄酮类化合物，且存在每种植物特有的植物化合物簇。因此，[具体植物1]和[具体植物2]是针对医学上关注的黄病毒的天然且安全的抗病毒植物化合物的潜在来源。
