Elevated CO Alters the Physiological and Transcriptome Responses of to Long-Term CO Exposure.

Physiological response and transcriptome changes were observed to investigate the effects on the growth, metabolism and genetic changes of grown for a long time in an environment with an elevated atmospheric CO concentration. Pine trees were grown at ambient (400 ppm) and elevated (560 ppm and 720 ppm) CO concentrations for 10 years in open-top chambers. The content of nonstructural carbohydrates was significantly increased in elevated CO. It was notable that the contents of chlorophylls significantly decreased at an elevated CO. The activities of antioxidants were significantly increased at an elevated CO concentration of 720 ppm. We analyzed the differences in the transcriptomes of at ambient and elevated CO concentrations and elucidated the functions of the differentially expressed genes (DEGs). RNA-Seq analysis identified 2415 and 4462 DEGs between an ambient and elevated CO concentrations of 560 ppm and 720 ppm, respectively. Genes related to glycolysis/gluconeogenesis and starch/sucrose metabolism were unchanged or decreased at an elevated CO concentration of 560 ppm and tended to increase at an elevated CO concentration of 720 ppm. It was confirmed that the expression levels of genes related to photosynthesis and antioxidants were increased at an elevated CO concentration of 720 ppm.