Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai, People's Republic of China.
Colloids Surf B Biointerfaces. 2012 Jan 1;89:254-64. doi: 10.1016/j.colsurfb.2011.09.029. Epub 2011 Sep 21.
We report the fabrication of water-stable electrospun γ-polyglutamic acid (γ-PGA) nanofibers with morphology control for biomedical applications. In this study, the processing variables including polymer concentration, flow rate, applied voltage, collection distance, and ambient humidity were systematically optimized to generate uniform γ-PGA nanofibers with a smooth morphology. By changing the trifluoroacetic acid concentration in the electrospinning solution, the diameter of the γ-PGA nanofibers can be controlled within the range of 186-603 nm. To render the γ-PGA nanofibers with good water stability, cystamine was employed as a crosslinking agent to amidate the carboxyl groups of γ-PGA. Furthermore, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay in conjunction of cell morphology observation reveals that the obtained γ-PGA nanofibers have an excellent biocompatibility to promote the cell adhesion and proliferation. We anticipate that the fabricated electrospun γ-PGA nanofibers with controllable morphology and good water stability may find extensive applications in future development of tissue engineering scaffold materials, drug delivery systems, environmental remediation, and sensing.
我们报告了一种用于生物医学应用的水稳定电纺γ-聚谷氨酸（γ-PGA）纳米纤维的制造，具有形态控制。在这项研究中，系统地优化了包括聚合物浓度、流速、施加电压、收集距离和环境湿度在内的加工变量，以生成具有光滑形态的均匀γ-PGA 纳米纤维。通过改变电纺溶液中的三氟乙酸浓度，可以将 γ-PGA 纳米纤维的直径控制在 186-603nm 范围内。为了使 γ-PGA 纳米纤维具有良好的水稳定性，胱胺被用作交联剂，使 γ-PGA 的羧基酰胺化。此外，3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴化物比色法结合细胞形态观察表明，所得到的 γ-PGA 纳米纤维具有优异的生物相容性，可促进细胞黏附和增殖。我们预计，具有可控形态和良好水稳定性的制备的电纺γ-PGA 纳米纤维可能在组织工程支架材料、药物传递系统、环境修复和传感等领域的未来发展中得到广泛应用。
