School of Medicine, Institute of Clinical Medicine, Pathology and Forensic Medicine, and Translational Cancer Research Area, University of Eastern Finland, Yliopistonranta 1 C, 70210, Kuopio, Finland.
School of Medicine, Institute of Clinical Medicine, University of Eastern Finland, Kuopio, Finland.
Sci Rep. 2022 Dec 30;12(1):22614. doi: 10.1038/s41598-022-26954-w.
Precision medicine approaches are required for more effective therapies for cancer. As small non-coding RNAs (sncRNAs) have recently been suggested as intriguing candidates for cancer biomarkers and have shown potential also as novel therapeutic targets, we aimed at profiling the non-miRNA sncRNAs in a large sample set to evaluate their role in invasive breast cancer (BC). We used small RNA sequencing and 195 fresh-frozen invasive BC and 22 benign breast tissue samples to identify significant associations of small nucleolar RNAs, small nuclear RNAs, and miscellaneous RNAs with the clinicopathological features and patient outcome of BC. Ninety-six and five sncRNAs significantly distinguished (Padj < 0.01) invasive local BC from benign breast tissue and metastasized BC from invasive local BC, respectively. Furthermore, 69 sncRNAs significantly associated (Padj < 0.01) with the tumor grade, hormone receptor status, subtype, and/or tumor histology. Additionally, 42 sncRNAs were observed as candidates for prognostic markers and 29 for predictive markers for radiotherapy and/or tamoxifen response (P < 0.05). We discovered the clinical relevance of sncRNAs from each studied RNA type. By introducing new sncRNA biomarker candidates for invasive BC and validating the potential of previously described ones, we have guided the way for further research that is warranted for providing novel insights into BC biology.
精准医疗方法是癌症更有效治疗的必要手段。由于小非编码 RNA（sncRNA）最近被认为是癌症生物标志物的有趣候选物，并显示出作为新型治疗靶点的潜力，我们旨在对大量样本集中的非 miRNA sncRNA 进行分析，以评估其在浸润性乳腺癌（BC）中的作用。我们使用小 RNA 测序和 195 份新鲜冷冻的浸润性 BC 和 22 份良性乳腺组织样本，以鉴定小核仁 RNA、小核 RNA 和其他 RNA 与 BC 的临床病理特征和患者预后的显著关联。有 96 种和 5 种 sncRNA 分别显著区分（Padj<0.01）浸润性局部 BC 与良性乳腺组织和转移性 BC 与浸润性局部 BC。此外，有 69 种 sncRNA 与肿瘤分级、激素受体状态、亚型和/或肿瘤组织学显著相关（Padj<0.01）。此外，观察到 42 种 sncRNA 作为预后标志物候选物，29 种作为放射治疗和/或他莫昔芬反应的预测标志物候选物（P<0.05）。我们发现了每种研究 RNA 类型的 sncRNA 的临床相关性。通过引入新的浸润性 BC sncRNA 生物标志物候选物并验证先前描述的候选物的潜力，我们为进一步的研究指明了方向，这对于为 BC 生物学提供新的见解是必要的。
Zotero 插件