Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University - Ain Helwan, Helwan, 11795, Egypt.
Curr Microbiol. 2023 Jan 2;80(2):57. doi: 10.1007/s00284-022-03151-6.
Infectious diseases remain one of the major health challenges worldwide due to the problem of antimicrobial resistance. Conventional antimicrobials have the disadvantage that bacteria rapidly acquire resistance to them, so alternatives must be developed to combat antibiotic resistance. Nanotechnology and the repurposing of existing drugs with known biological profiles are new approaches to replacing conventional antimicrobials. In this paper, we have tested the antibacterial activity of sodium acetate (NaA), vitamin C (VC), and zinc oxide nanoparticles (ZnO NPs) against Escherichia coli O157:H7 ATCC 51659 and Pseudomonas aeruginosa ATCC 27853. MIC values for tested compounds ranged from 0.08 to 6.5 mg ml, and the effect of combinations and safety profiles against HepG2 cell line of these compounds were also evaluated. At sub-MIC values, tested compounds had a potential antivirulence effect by inhibiting motility and reducing biofilm formation and maturation. Collectively, ZnO NPs and VC are considered safe alternatives to traditional antibiotics that are capable of reducing the development of antibiotic resistance in microbes. Graphical abstract representing the main aim and the final findings of our work. Spread of multidrug-resistant (MDR) bacterial strains created an urge for alternative safe antimicrobial agents. In this work, we found that ZnO NPs and vitamin C are potential candidates that could be used against MDR E.coli and P. aeruginosa.
由于抗菌药物耐药性的问题，传染病仍然是全球主要的健康挑战之一。传统的抗菌药物有一个缺点，就是细菌会迅速对其产生耐药性，因此必须开发替代品来对抗抗生素耐药性。纳米技术和具有已知生物学特性的现有药物的再利用是替代传统抗菌药物的新方法。在本文中，我们测试了乙酸钠（NaA）、维生素 C（VC）和氧化锌纳米颗粒（ZnO NPs）对大肠杆菌 O157:H7 ATCC 51659 和铜绿假单胞菌 ATCC 27853 的抗菌活性。测试化合物的 MIC 值范围为 0.08 至 6.5 mg/ml，还评估了这些化合物的组合效果和对 HepG2 细胞系的安全性。在亚 MIC 值下，测试化合物通过抑制运动性、减少生物膜形成和成熟，具有潜在的抗毒力作用。总之，ZnO NPs 和 VC 被认为是传统抗生素的安全替代品，能够减少微生物对抗生素耐药性的发展。
