Ilyas Syafruddin, Simanullang Rostime Hermayerni, Hutahaean Salomo, Rosidah Rosidah, Situmorang Putri Cahaya
Pak J Biol Sci. 2022 Jan;25(11):1014-1020. doi: 10.3923/pjbs.2022.1014.1020.
<b>Background and Objective:</b> Natural herbs and molecular therapy can be used to treat cervical cancer. The Myc and Wee1 control tumour cell fate and microenvironmental changes like angiogenesis activation and host immune response suppression. The study aims to know about the correlation of Myc and Wee1 expressions as a molecular therapy given by <i>Zanthoxylum acanthopodium</i>. <b>Materials and Methods:</b> There are five rat groups: Group K<sup></sup> is the untreated group, Group K<sup>+</sup> is the rats injected with benzopyrene, Group P<sub>1</sub> is the administration of <i>Zanthoxylum acanthopodium</i> 100 mg kg<sup>1</sup> b.wt., Group P<sub>2</sub> is the administration of <i>Zanthoxylum acanthopodium</i> 200 mg kg<sup>1</sup> b.wt. and Group P<sub>3</sub> is the administration of <i>Zanthoxylum acanthopodium</i> 400 mg kg<sup>1</sup> b.wt. The rats are dissected 30 days after receiving <i>Zanthoxylum acanthopodium</i>. To stain the cervical tissues, immunohistochemistry is performed. <b>Results:</b> <i>Zanthoxylum acanthopodium</i> administration caused epithelial thickening and decreased Myc expression in previously uncontrolled carcinomas from untreated malignancies, which now slowed and stopped growing into the normal epithelium. Wee1 expression revealed that this herb could repair tissue by drastically reducing Wee1 expression at a dose of 100-400 mg kg<sup>1</sup> b.wt. Similarly, at the highest dose, cervical carcinoma stops growing and the nucleus begins to form normally (p<0.01). <b>Conclusion:</b> The higher Myc expression on andaliman administration in cervical carcinoma decreases Wee1 expression in cervical carcinoma so these two proteins have a strong and significant correlation. <i>Zanthoxylum acanthopodium</i> can be administered at various dosages to lower the number of positive indexes of Myc and Wee1 expression in cervical carcinoma.
背景与目的：天然草药和分子疗法可用于治疗宫颈癌。Myc和Wee1控制肿瘤细胞命运以及诸如血管生成激活和宿主免疫反应抑制等微环境变化。本研究旨在了解作为一种分子疗法的刺花椒所给予的Myc和Wee1表达之间的相关性。材料与方法：有五个大鼠组：K组为未治疗组，K+组为注射苯并芘的大鼠组，P1组给予刺花椒100 mg/kg体重，P2组给予刺花椒200 mg/kg体重，P3组给予刺花椒400 mg/kg体重。在给予刺花椒30天后解剖大鼠。采用免疫组织化学法对宫颈组织进行染色。结果：给予刺花椒导致上皮增厚，并使先前未受控制的未治疗恶性肿瘤中的癌组织Myc表达降低，现在其生长减缓并停止向正常上皮组织生长。Wee1表达显示，该草药在剂量为100 - 400 mg/kg体重时可通过大幅降低Wee1表达来修复组织。同样，在最高剂量时，宫颈癌停止生长且细胞核开始正常形成（p<0.01）。结论：在宫颈癌中给予刺花椒时较高的Myc表达会降低宫颈癌中的Wee1表达，因此这两种蛋白具有强烈且显著的相关性。刺花椒可采用不同剂量给药以降低宫颈癌中Myc和Wee1表达的阳性指数数量。
