Pak J Biol Sci. 2022 Jan;25(1):29-36. doi: 10.3923/pjbs.2022.29.36.
<b>Background and Objective:</b> Cervical cancer is the leading cause of death for women in the world and Indonesia. This disease originates from a malignant tumour of squamous epithelial cells caused by infection with the Human Papilloma Virus (HPV). Antioxidants can reduce oxidative stress in and there are plants from Indonesia that have high antioxidants, namely andaliman (<i>Zanthoxylum acanthopodium</i>). This study aimed to analyze the role of andaliman on PI3K and Wnt signalling in cervical cancer histology. <b>Materials and Methods:</b> The study includes 5 treatments. The control group (K-), rats cancer model (K+), rats cancer model+the dose is 100 mg/b.wt. of ZAM (P<sub>1</sub>), rats cancer model+the dose is 200 mg/b.wt. of ZAM (P<sub>2</sub>) and rats cancer model+the dosage is 400 mg/b.wt. ZAM (P<sub>3</sub>). On the 30th day after ZAM administration, the rats were dissected for the paraffin block and Wnt and PI3K immunohistochemical staining was prepared. <b>Results:</b> There was a significant difference between all groups (p<0.001) in Wnt and PI3K expression. The real role of ZAM in cervical cancer tissue was seen at the highest ZAM dose (P<sub>3</sub>). Irregular mucosal folds and stretched interstitial connective tissue in the K+ group can return to regularity and improve at the P<sub>3</sub> dose. The administration of ZAM showed a significant difference in cervical tissue after benzopyrene injection. <b>Conclusion:</b> Andaliman (<i>Zanthoxylum acanthopodium</i>) extract increases PI3K expression through suppression of Wnt expression. It can be developed therapy molecularly to prevent cell growth into cancer.
宫颈癌是全球和印度尼西亚女性死亡的主要原因。这种疾病源于鳞状上皮细胞的恶性肿瘤，由人乳头瘤病毒（HPV）感染引起。抗氧化剂可以减轻氧化应激，印度尼西亚有一些植物具有很高的抗氧化剂，即酸豆（<i>Zanthoxylum acanthopodium</i>）。本研究旨在分析酸豆对宫颈癌组织中 PI3K 和 Wnt 信号的作用。
本研究共包含 5 种处理。对照组（K-）、大鼠宫颈癌模型（K+）、大鼠宫颈癌模型+ZAM 剂量为 100mg/b.wt.（P<sub>1</sub>）、大鼠宫颈癌模型+ZAM 剂量为 200mg/b.wt.（P<sub>2</sub>）和大鼠宫颈癌模型+ZAM 剂量为 400mg/b.wt.（P<sub>3</sub>）。ZAM 给药后第 30 天，对大鼠进行解剖，制作石蜡块，并进行 Wnt 和 PI3K 免疫组织化学染色。
各组间 Wnt 和 PI3K 表达均有显著差异（p<0.001）。在 ZAM 剂量最高时（P<sub>3</sub>），观察到 ZAM 对宫颈癌组织的实际作用。K+组不规则的黏膜皱襞和伸展的间质结缔组织在 P<sub>3</sub>剂量下可恢复正常。ZAM 给药后，苯并芘注射后的宫颈组织有显著差异。
酸豆（<i>Zanthoxylum acanthopodium</i>）提取物通过抑制 Wnt 表达增加 PI3K 表达。它可以作为一种预防细胞癌变的分子治疗方法进行开发。
