Department of Chemical Engineering, University of Almeria, 04120 Almeria, Spain; CIESOL Solar Energy Research Centre, Joint Centre University of Almeria-CIEMAT, 04120 Almeria, Spain.
Department of Chemical Engineering, University of Almeria, 04120 Almeria, Spain.
Food Res Int. 2023 Jan;163:112270. doi: 10.1016/j.foodres.2022.112270. Epub 2022 Dec 7.
The microalga Arthrospira platensis BEA 005B was produced using 80 m (9 m) raceway photobioreactors achieving a biomass productivity of 28.2 g·m·day when operating the reactors in semi-continuous mode (0.33 day). The produced biomass was rich in proteins (58.1 g·100 g) and carbohydrates (25.6 g·100 g); the content of phycocyanins and allophycocyanins was 115.4 and 36.9 mg·g, respectively. Ultrasounds and high-pressure homogenisation allowed recovering approximately 90% of the initial protein content of the biomass; however, the energetic requirements of the former (∼100 kJ·kg) were significantly lower than those of high-pressure homogenisation (∼200 kJ·kg). An in silico analysis revealed that papain and ficin would allow releasing a large number of bioactive peptides with antioxidant, antihypertensive (ACE-I and renin), and antidiabetic (DPP-IV, α-amylase, and α-glucosidase) properties. Both were assessed in vitro together with Alcalase and pepsin leading to the generation of enzymatic hydrolysates with in vitro bioactivity.
小球藻 Arthrospira platensis BEA 005B 采用 80 m（9 m）跑道式光生物反应器生产，当以半连续模式（0.33 天）运行反应器时，生物量生产力达到 28.2 g·m·day。所生产的生物质富含蛋白质（58.1 g·100 g）和碳水化合物（25.6 g·100 g）；藻蓝蛋白和别藻蓝蛋白的含量分别为 115.4 和 36.9 mg·g。超声和高压匀浆允许回收约 90%的生物质初始蛋白质含量；然而，前者（约 100 kJ·kg）的能量需求明显低于高压匀浆（约 200 kJ·kg）。计算机分析表明，木瓜蛋白酶和无花果蛋白酶可释放大量具有抗氧化、降血压（ACE-I 和肾素）和抗糖尿病（DPP-IV、α-淀粉酶和α-葡萄糖苷酶）特性的生物活性肽。这两种酶与 Alcalase 和胃蛋白酶一起在体外进行了评估，导致产生具有体外生物活性的酶解产物。
