5-溴脱氧尿苷对大鼠肝癌细胞不均一核RNA的影响。
Effect of 5-bromodeoxyuridine on heterogeneous nuclear RNA in rat hepatoma cells.
作者信息
Weber H W, Stellwagen R H
出版信息
Nucleic Acids Res. 1979 Apr;6(4):1731-46. doi: 10.1093/nar/6.4.1731.
Heterogeneous nuclear RNA HnRNA) was isolated from untreated and 5-bromodeoxyuridine (BrdUrd) treated hepatoma tissue culture (HTC) cells. analysis of this RNA by either electrophoresis on polyacrylamide-agarose gels or centrifugation in sucrose gradients demonstrated that BrdUrd caused a shift in the labeled HnRNA population toward a smaller size distribution. This effect was produced by concentrations of BrdUrd which specifically lower the level of the differentiated enzyme tyrosine aminotransferase, but do not greatly affect cell growth. Differential binding to oligo(dT) cellulose was used to fractionate HnRNA further into classes containing poly(A) (alpha), oligo(A) (beta) or neither category of A-rich sequences (gamma). BrdUrd did not alter the relative rates of uridine incorporation into the three classes. The shift in the labeled HnRNA population due to BrdUrd was observed in all three subclasses of HnRNA.
从未经处理和经5-溴脱氧尿苷（BrdUrd）处理的肝癌组织培养（HTC）细胞中分离出不均一核RNA（HnRNA）。通过在聚丙烯酰胺-琼脂糖凝胶上电泳或在蔗糖梯度中离心对该RNA进行分析，结果表明BrdUrd使标记的HnRNA群体向更小的大小分布转移。产生这种效应的BrdUrd浓度可特异性降低分化酶酪氨酸转氨酶的水平，但对细胞生长影响不大。利用与寡聚（dT）纤维素的差异结合，将HnRNA进一步分为含有多聚（A）（α）、寡聚（A）（β）或既不含有富含A序列这两类中的任何一类（γ）的类别。BrdUrd并未改变尿苷掺入这三类中的相对速率。在HnRNA的所有三个亚类中均观察到由于BrdUrd导致的标记HnRNA群体的转移。
Zotero 插件