Li Rong, Yuan Xin, Zhang Li, Jiang Xuebing, Li Li, Zhang Yi, Guo Linghong, Dai Xide, Cheng Hao, Jiang Xian, Gou Maling
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, 610041, Chengdu, China.
Department of Plastic and Burn Surgery, West China Hospital, Sichuan University, 610041, Chengdu, China.
Mol Biomed. 2023 Jan 5;4(1):1. doi: 10.1186/s43556-022-00102-2.
Hair loss is a common skin disease that causes intense emotional suffering. Hair regeneration in a personalized area is highly desirable for patients with different balding conditions. However, the existing pharmaceutical treatments have difficulty precisely regenerating hair in a desired area. Here, we show a method to precisely control the hair regeneration using customized microneedle arrays (MNAs). The MNA with a customized shape is fast fabricated by a static optical projection lithography process in seconds, which is a 3D printing technology developed by our group. In the mouse model, MNA treatment could induce hair regrowth in a defined area corresponding to the customized shape of MNA. And the regenerated hair promoted by MNAs had improved quality. Cellular and molecular analysis indicated that MNA treatment could recruit macrophages in situ and then initiate the proliferation of hair follicle stem cells, thereby improving hair regeneration. Meanwhile, the activation of the Wnt/β-catenin signaling pathway was observed in hair follicles. The expressions of Hgf, Igf 1 and Tnf-α were also upregulated in the treated skin, which may also be beneficial for the MNA-induced hair regeneration. This study provides a strategy to precisely control hair regeneration using customized microneedle arrays by recruiting macrophages in situ, which holds the promise for the personalized treatment of hair loss.
脱发是一种常见的皮肤病，会导致强烈的情感痛苦。对于不同脱发情况的患者来说，在个性化区域实现毛发再生是非常理想的。然而，现有的药物治疗难以在期望的区域精确地再生毛发。在此，我们展示了一种使用定制微针阵列（MNAs）精确控制毛发再生的方法。具有定制形状的微针阵列通过静态光学投影光刻工艺在数秒内快速制造而成，这是我们团队开发的一种3D打印技术。在小鼠模型中，微针阵列治疗能够在与微针阵列定制形状相对应的特定区域诱导毛发再生。并且由微针阵列促进再生的毛发质量有所改善。细胞和分子分析表明，微针阵列治疗能够在原位募集巨噬细胞，然后启动毛囊干细胞的增殖，从而改善毛发再生。同时，在毛囊中观察到Wnt/β-连环蛋白信号通路的激活。在治疗后的皮肤中，Hgf、Igf 1和Tnf-α的表达也上调，这可能也有利于微针阵列诱导的毛发再生。本研究提供了一种通过原位募集巨噬细胞使用定制微针阵列精确控制毛发再生的策略，这为脱发的个性化治疗带来了希望。
