空间 ATAC 进行固相捕获和开放染色质分析。
Solid-phase capture and profiling of open chromatin by spatial ATAC.
机构信息
Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
SciLifeLab, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.
出版信息
Nat Biotechnol. 2023 Aug;41(8):1085-1088. doi: 10.1038/s41587-022-01603-9. Epub 2023 Jan 5.
Abstract
Current methods for epigenomic profiling are limited in their ability to obtain genome-wide information with spatial resolution. We introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially resolved epigenomics. We show that spatial ATAC enables the discovery of the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation and in human pathology.
摘要
目前的表观基因组分析方法在获取具有空间分辨率的全基因组信息方面能力有限。我们介绍了空间 ATAC 方法，该方法将组织切片中的转座酶可及染色质分析与带有条形码的固相捕获相结合，以进行空间分辨的表观基因组学研究。我们表明，空间 ATAC 能够发现小鼠器官发生、谱系分化和人类病理学过程中空间基因表达的调控程序。
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