Birang Reza, Shakerian Kourosh, Birang Ehsan, Narimani Tahmineh, Naghsh Narges, Fekrazad Reza
Department of Periodontology, Dental Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Periodontology, School of Dentistry, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Dent Res J (Isfahan). 2022 Dec 14;19:111. eCollection 2022.
Decontaminating the implant surface, exposed to bacterial biofilm, is a concern in the treatment of peri-implant inflammatory disease. The aim of this study was to compare the effect of several methods on reduction of the bacterial load, colonized on the surfaces of titanium discs.
In this study, seven titanium discs with Sandblasted, Large-grit, acid-etched (SLA) surface were placed in the mouth of each of ten patients with chronic periodontitis by an intra-oral maxillary splint for 24 h. In each patient, the contaminated discs, except for the negative control ones, were randomly treated by one of the six antiseptic methods including sterile normal saline, plastic curette, air polisher, hydrogen peroxide, 980 nm diode laser, and Er-YAG laser. A spectrophotometer was used to measure Optical Density (OD) in case of aerobic microorganisms. Colony-Forming Units (CFUs) were used for anaerobic bacteria. Data were analyzed through Kruskal-Wallis and Mann-Whitney Tests at a significance level of α =0.05 by SPSS software.
Statistical analysis showed a significant decrease in OD of aerobic bacteria among the seven groups during a 0-24 h time interval ( < 0.001). Furthermore, these tests showed a significant difference in the CFU ( < 0.001) for anaerobic bacteria after 48 h.
The results of this study showed that all of the adopted methods significantly reduced microbial colonies on the surfaces of titanium discs with SLA surface. Er: YAG laser and normal saline had the highest and the lowest effects, respectively.
对暴露于细菌生物膜的种植体表面进行去污处理是种植体周围炎症性疾病治疗中的一个关注点。本研究的目的是比较几种方法对减少钛盘表面定植细菌载量的效果。
在本研究中，通过口内上颌夹板将七个具有喷砂、大颗粒、酸蚀（SLA）表面的钛盘放置在十名慢性牙周炎患者的口中24小时。在每位患者中，除阴性对照盘外，受污染的盘随机采用六种抗菌方法之一进行处理，包括无菌生理盐水、塑料刮匙、空气抛光器、过氧化氢、980纳米二极管激光和铒钇铝石榴石激光。对于需氧微生物，使用分光光度计测量光密度（OD）。对于厌氧细菌，使用菌落形成单位（CFU）。数据通过SPSS软件进行Kruskal-Wallis和Mann-Whitney检验分析，显著性水平为α = 0.05。
统计分析表明，在0 - 24小时时间间隔内，七组中需氧细菌的OD显著降低（< 0.001）。此外，这些测试表明，48小时后厌氧细菌的CFU存在显著差异（< 0.001）。
本研究结果表明，所有采用的方法均显著减少了具有SLA表面的钛盘表面的微生物菌落。铒钇铝石榴石激光和生理盐水的效果分别最高和最低。
