Lu Yingjie, Cao Yuqi, Chen Danqing, Zhou Yaobin, Zhang Li, Su Yue, Guo Yinlong
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
State Key Laboratory of Organometallic Chemistry and National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, PR China.
Food Chem. 2023 Jun 1;410:135365. doi: 10.1016/j.foodchem.2022.135365. Epub 2022 Dec 30.
Serving as a world-renowned tonic, ginseng contains various types of bioactive metabolites. The comprehensive profiling of these metabolites may help explore the nutritional value of ginseng. Due to high variety in chemical structures, simultaneous monitoring of these metabolites remains a challenge. Herein, a high-throughput and high-selectivity online derivatization mass spectrometry imaging strategy targeting CC was developed. As a widely existed chemical group, CC acts like a bridge connecting different kinds of metabolites. [d]/[d]-Bis(pyridine) iodine tetrafluoroboride reagent was chosen for the derivatization of CC, the detection sensitivity of which increased about 3 magnitudes after derivatization. Assisted by laser ablation carbon fiber ionization mass spectrometry, the spatial distribution of bioactive metabolites in mountain-cultivated and garden-cultivated ginseng were visualized. The correlation heatmap results revealed that metabolites in mountain-cultivated ginseng hold higher correlation than those in garden-cultivated ginseng. The proposed method showed potential in providing comprehensive information on the nutrient content of foods.
人参作为一种世界闻名的滋补品，含有多种生物活性代谢物。对这些代谢物进行全面分析有助于探索人参的营养价值。由于化学结构种类繁多，同时监测这些代谢物仍然是一项挑战。在此，开发了一种针对共轭羰基（CC）的高通量、高选择性在线衍生化质谱成像策略。作为一种广泛存在的化学基团，CC就像一座连接不同种类代谢物的桥梁。选择[氘代]/[氘代] - 双（吡啶）四氟硼酸碘试剂对CC进行衍生化，衍生化后其检测灵敏度提高了约3个数量级。在激光烧蚀碳纤维电离质谱的辅助下，可视化了山地种植和园圃种植人参中生物活性代谢物的空间分布。相关热图结果显示，山地种植人参中的代谢物比园圃种植人参中的代谢物具有更高的相关性。所提出的方法在提供食品营养成分综合信息方面显示出潜力。
