Bishop D H, Gould K G, Akashi H, Clerx-van Haaster C M
Nucleic Acids Res. 1982 Jun 25;10(12):3703-13. doi: 10.1093/nar/10.12.3703.
The complete sequence of the small (S) viral RNA species of snowshoe hare (SSH) bunyavirus has been determined, principally from a DNA copy of the RNA cloned in the E.coli plasmid pBr322. The viral S RNA (negative sense strand) is 982 nucleotides long (3.3 x 10(5) daltons) with complementary 5' and 3' end sequences. It has a base composition of 30.5%U, 25.8%A, 24.9%C and 18.7%G. In the viral complementary (plus sense) strand there are two overlapping open reading frames initiated by methionine codons. One reading frame codes for a 26.8 x 10(3) dalton protein, the other for a 10.5 x 10(3) dalton protein. The larger gene product is presumably related to the viral nucleoprotein (N) that is coded by the S RNA (Gentsch and Bishop (1978) J. Virol. 28, 417-419). The smaller gene product is probably related to the recently identified S RNA coded nonstructural protein (NSS) induced in virus infected cells (Fuller and Bishop (1982) J. Virol. 41, 643-648).
已确定了雪兔(SSH)布尼亚病毒小(S)病毒RNA种类的完整序列,主要来自克隆于大肠杆菌质粒pBr322中的RNA的DNA拷贝。病毒S RNA(负义链)长982个核苷酸(3.3×10⁵道尔顿),5'和3'末端序列互补。其碱基组成为30.5%U、25.8%A、24.9%C和18.7%G。在病毒互补(正义)链中有两个由甲硫氨酸密码子起始的重叠开放阅读框。一个阅读框编码一个26.8×10³道尔顿的蛋白质,另一个编码一个10.5×10³道尔顿的蛋白质。较大的基因产物可能与由S RNA编码的病毒核蛋白(N)相关(Gentsch和Bishop(1978年)《病毒学杂志》28卷,417 - 419页)。较小的基因产物可能与最近在病毒感染细胞中诱导产生的、由S RNA编码的非结构蛋白(NSS)相关(Fuller和Bishop(1982年)《病毒学杂志》41卷,643 - 648页)。