Fuller F, Bhown A S, Bishop D H
J Gen Virol. 1983 Aug;64 (Pt 8):1705-14. doi: 10.1099/0022-1317-64-8-1705.
It has been shown previously, by sequence analysis of the S RNA segment of snowshoe hare (SSH) bunyavirus, that two overlapping open reading frames in the viral complementary sequence code for proteins with molecular weights of 26.8 X 10(3) and 10.5 X 10(3) respectively. In addition to the viral nucleocapsid (N) protein, which is coded by the S RNA, analyses of parental and reassortant bunyavirus-infected cell extracts have shown that the viral S RNA and M RNA species each code for non-structural proteins (NSS and NSM, respectively). In the present report, in vitro translation analyses of the S mRNA species recovered from virus-infected cells indicate that a single size class of mRNA directs the synthesis of N and NSS. Compositional analyses of selected tryptic peptides of N and NSS have provided proof that N is the product of the first open reading frame, and NSS the product of the second.
先前通过对雪兔（SSH）布尼亚病毒S RNA片段的序列分析表明，病毒互补序列中的两个重叠开放阅读框分别编码分子量为26.8×10³和10.5×10³的蛋白质。除了由S RNA编码的病毒核衣壳（N）蛋白外，对亲本和重配布尼亚病毒感染细胞提取物的分析表明，病毒S RNA和M RNA种类分别编码非结构蛋白（分别为NSS和NSM）。在本报告中，对从病毒感染细胞中回收的S mRNA种类的体外翻译分析表明，单一大小类别的mRNA指导N和NSS的合成。对N和NSS选定胰蛋白酶肽的组成分析证明，N是第一个开放阅读框的产物，NSS是第二个开放阅读框的产物。
