Akashi H, Bishop D H
J Virol. 1983 Mar;45(3):1155-8. doi: 10.1128/JVI.45.3.1155-1158.1983.
The sequence of the S RNA of La Crosse bunyavirus was deduced from analyses of DNA copies cloned in the Escherichia coli plasmid pBR322. The S RNA is 984 nucleotides in length, has a base ratio of 31.8% U, 27.0% A, 23.2% C, and 18.0% G, and codes for two distinct gene products that are read from overlapping reading frames in the viral complementary strand. The larger gene product (N, 26.5 x 10(3) daltons) contains 235 amino acids, and the smaller gene product (NSS, 10.4 x 10(3) daltons) has 92 amino acids. Comparisons with the published sequences of the related snowshoe hare bunyavirus S RNA and its gene products (Bishop et al., Nucleic Acids Res. 10:3703-3713, 1982) indicate that there are a total of 114 nucleotide differences (6 additions or deletions and 108 substitutions). Also, there are 22 amino acid differences between the N proteins and 12 amino acid differences between the NSS proteins of the two S RNAs.
通过对克隆于大肠杆菌质粒pBR322中的DNA拷贝进行分析，推导得出了拉克罗斯布尼亚病毒S RNA的序列。S RNA长度为984个核苷酸，碱基比例为：尿嘧啶（U）31.8%、腺嘌呤（A）27.0%、胞嘧啶（C）23.2%、鸟嘌呤（G）18.0%，并编码两种不同的基因产物，这两种产物由病毒互补链中的重叠阅读框转录而来。较大的基因产物（N，26.5×10³道尔顿）包含235个氨基酸，较小的基因产物（NSS，10.4×10³道尔顿）有92个氨基酸。与已发表的相关雪兔布尼亚病毒S RNA及其基因产物的序列（毕晓普等人，《核酸研究》10:3703 - 3713, 1982）进行比较表明，总共有114个核苷酸差异（6个插入或缺失以及108个替换）。此外，两种S RNA的N蛋白之间有22个氨基酸差异，NSS蛋白之间有12个氨基酸差异。
