Department of Biochemistry, Punjab Agricultural University, Punjab, India.
Department of Plant Breeding and Genetics, Punjab Agricultural University, Punjab, India.
Biol Futur. 2023 Jun;74(1-2):231-246. doi: 10.1007/s42977-022-00151-2. Epub 2023 Jan 7.
Five desi (GL 12,021, GL 29,095, GL 29,078, H11 22 and CSJ 515) and three wild (GLW 22, GLW 58 and GLW 187) chickpea cultivars showed induced defense response against Helicoverpa armigera infestation as a result of enhanced activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, glutathione reductase, polyphenol oxidase, phenylalanine ammonia lyase, tyrosine ammonia lyase in leaves, pod walls and seeds. Catalase activity increased in leaves of GL 12,021, H11 22, GL 29,095, CSJ 515, GLW 22, and GL 29,078 after infestation compared to resistant check; catalase and peroxidase activities in GL 29,095 and GL 29,078; ascorbate peroxidase and glutathione reductase activities in leaves of GLW 58. The increased activity of superoxide dismutase in pod wall of H1122; catalase in pod wall of 29,078, GL 29,095 and GL 22; ascorbate peroxidase and glutathione reductase in pod wall of GLW 58; phenylalanine ammonia lyase and tyrosine ammonia lyase in pod wall of GLW 187, H11 22, GL 20,978, GLW 22 and GLW 58 after infestation as compared to resistant check might be responsible for mitigating infestation induced oxidative stress. MDA content decreased in leaves, pod wall and seeds of GLW 187 and GL 12,021 after infestation. Lower percent pod damage (9.58-12.44%) in GL 12,021, GLW 187, GL 29,095, H11 22, GL 29,078, GLW 22 and GLW 58 as compared to resistant (16.18%) and susceptible (21.50) checks might be attributed to differential induced defense mechanism in them. The identified desi and wild genotypes might be used in breeding program to develop cultivars with improved resistance to herbivore.
五种栽培（GL 12,021、GL 29,095、GL 29,078、H11 22 和 CSJ 515）和三种野生（GLW 22、GLW 58 和 GLW 187）鹰嘴豆品种在受到棉铃虫侵害后表现出诱导的防御反应，导致叶片、豆荚壁和种子中超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶、多酚氧化酶、苯丙氨酸解氨酶、酪氨酸解氨酶活性增强。与抗性对照相比，受侵害后 GL 12,021、H11 22、GL 29,095、CSJ 515、GLW 22 和 GL 29,078 的叶片中过氧化氢酶活性增加；GL 29,095 和 GL 29,078 的过氧化氢酶和过氧化物酶活性增加；GLW 58 的叶片中抗坏血酸过氧化物酶和谷胱甘肽还原酶活性增加。H1122 豆荚壁中超氧化物歧化酶活性增加；29,078、GL 29,095 和 GL 22 的豆荚壁中过氧化氢酶活性增加；GLW 58 的豆荚壁中抗坏血酸过氧化物酶和谷胱甘肽还原酶活性增加；受侵害后 GLW 187、H11 22、GL 20,978、GLW 22 和 GLW 58 的豆荚壁中苯丙氨酸解氨酶和酪氨酸解氨酶活性增加，与抗性对照相比，可能有助于减轻侵害引起的氧化应激。受侵害后 GLW 187 和 GL 12,021 的叶片、豆荚壁和种子中的 MDA 含量降低。与抗性对照（16.18%）和感病对照（21.50%）相比，GL 12,021、GLW 187、GL 29,095、H11 22、GL 29,078、GLW 22 和 GLW 58 的豆荚损伤率（9.58-12.44%）较低，这可能归因于它们之间不同的诱导防御机制。已鉴定的栽培和野生基因型可能用于育种计划，以开发对草食性动物具有改良抗性的品种。
