School of Pharmaceutical, East China University of Science and Technology, Shanghai 200237, China.
Shanghai Qingpu District Agricultural Technology Extension Service Center, Shanghai 201700, China.
Molecules. 2022 Dec 20;28(1):17. doi: 10.3390/molecules28010017.
A strain of marine actinomycetes was isolated from an intertidal zone and identified as . Through the fermentation of this strain, a compound with fungicidal activity was extracted and purified. Using mass spectrometry (MS) and nuclear magnetic resonance (NMR) data, the metabolite was determined to be an aurone. The toxicity of the aurone toward four kinds of tumor cells-SH-SY5Y, HepG2, A549, and HeLa cells-was verified by the MTT method, delivering IC values of 41.81, 47.19, 63.95, and 51.92 μg/mL, respectively. Greenhouse bioassay showed that the aurone exhibited a high fungicidal activity against powder mildew (), cucurbits powder mildew ( (Schlecht ex Ff.) Poll), and rice blast ().
从潮间带分离得到一株海洋放线菌，经鉴定为 。通过该菌株的发酵，提取并纯化得到一种具有杀菌活性的化合物。通过质谱（MS）和核磁共振（NMR）数据确定该代谢产物为一种橙酮。采用 MTT 法验证了橙酮对四种肿瘤细胞-SH-SY5Y、HepG2、A549 和 HeLa 细胞的毒性，其 IC 值分别为 41.81、47.19、63.95 和 51.92 μg/mL。温室生物测定表明，橙酮对瓜类白粉病（）、葫芦科白粉病（ (Schlecht ex Ff.) Poll）和稻瘟病（）具有较高的杀菌活性。
