Sghaier Nesrine, Ayed Rayda Ben, Rebai Ahmed
Laboratory of Advanced Technology and Intelligent Systems, National Engineering School of Sousse, Sousse 4023, Tunisia.
Department of Agronomy and Plant Biotechnology, National Institute of Agronomy of Tunisia (INAT), 43 Avenue Charles Nicolle, 1082 El Mahrajène, University of Carthage-Tunis, Tunisia.
Genomics Inform. 2022 Dec;20(4):e45. doi: 10.5808/gi.22056. Epub 2022 Dec 30.
Food security will be affected by climate change worldwide, particularly in the developingworld, where the most important food products originate from plants. Plants are often exposed to environmental stresses that may affect their growth, development, yield, and foodquality. Auxin is a hormone that plays a critical role in improving plants' tolerance of environmental conditions. Auxin controls the expression of many stress-responsive genes inplants by interacting with specific cis-regulatory elements called auxin-responsive elements (AuxREs). In this work, we performed an in silico prediction of AuxREs in promotersof five auxin-responsive genes in Zea mays. We applied a data fusion approach based onthe combined use of Dempster-Shafer evidence theory and fuzzy sets. Auxin has a directimpact on cell membrane proteins. The short-term auxin response may be represented bythe regulation of transmembrane gene expression. The detection of an AuxRE in the promoter of prolyl oligopeptidase (POP) in Z. mays and the 3-fold overexpression of this geneunder auxin treatment for 30 min indicated the role of POP in maize auxin response. POP isregulated by auxin to perform stress adaptation. In addition, the detection of two AuxRETGTCTC motifs in the upstream sequence of the bx1 gene suggests that bx1 can be regulated by auxin. Auxin may also be involved in the regulation of dehydration-responsive element-binding and some members of the protein kinase superfamily.
全球粮食安全将受到气候变化的影响，尤其是在发展中国家，那里最重要的粮食产品都源自植物。植物常常面临可能影响其生长、发育、产量和食品质量的环境胁迫。生长素是一种在提高植物对环境条件耐受性方面起关键作用的激素。生长素通过与称为生长素反应元件（AuxREs）的特定顺式调控元件相互作用，控制植物中许多胁迫响应基因的表达。在这项工作中，我们对玉米中五个生长素响应基因启动子中的AuxREs进行了计算机预测。我们应用了一种基于Dempster-Shafer证据理论和模糊集联合使用的数据融合方法。生长素对细胞膜蛋白有直接影响。生长素的短期反应可能由跨膜基因表达的调控来体现。在玉米脯氨酰寡肽酶（POP）启动子中检测到一个AuxRE，并且该基因在生长素处理30分钟后有3倍的过表达，这表明POP在玉米生长素反应中发挥作用。POP受生长素调控以进行胁迫适应。此外，在bx1基因上游序列中检测到两个AuxRE TGTCTC基序，这表明bx1可受生长素调控。生长素还可能参与脱水响应元件结合蛋白和蛋白激酶超家族一些成员的调控。
