Sarshar Meysam, Scribano Daniela, Palamara Anna Teresa, Ambrosi Cecilia, Masotti Andrea
Research Laboratories, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
Front Mol Biosci. 2022 Dec 21;9:1088783. doi: 10.3389/fmolb.2022.1088783. eCollection 2022.
Bacterial small RNAs (sRNAs) research has accelerated over the past decade, boosted by advances in RNA-seq technologies and methodologies for capturing both protein-RNA and RNA-RNA interactions. The emerging picture is that these regulatory sRNAs play important roles in controlling complex physiological processes and are required to survive the antimicrobial challenge. In recent years, the RNA content of OMVs/EVs has also gained increasing attention, particularly in the context of infection. Secreted RNAs from several bacterial pathogens have been characterized but the exact mechanisms promoting pathogenicity remain elusive. In this review, we briefly discuss how secreted sRNAs interact with targets in infected cells, thus representing a novel perspective of host cell manipulation during bacterial infection. During the last decade, became clinically relevant emerging pathogens responsible for nosocomial and community-acquired infections. Therefore, we also summarize recent findings of regulation by sRNAs in and discuss how this emerging bacterium utilizes many of these sRNAs to adapt to its niche and become successful human pathogen.
在过去十年中，细菌小RNA（sRNA）的研究加速发展，这得益于RNA测序技术以及捕获蛋白质-RNA和RNA-RNA相互作用方法的进步。新出现的情况是，这些调控性sRNA在控制复杂生理过程中发挥重要作用，并且是应对抗菌挑战所必需的。近年来，外膜囊泡/细胞外囊泡（OMV/EV）的RNA含量也越来越受到关注，尤其是在感染的背景下。几种细菌病原体分泌的RNA已得到表征，但促进致病性的确切机制仍然难以捉摸。在这篇综述中，我们简要讨论分泌的sRNA如何与感染细胞中的靶标相互作用，从而代表了细菌感染期间宿主细胞操纵的一种新视角。在过去十年中， 成为导致医院感染和社区获得性感染的具有临床相关性的新兴病原体。因此，我们还总结了sRNA在 中的调控的最新发现，并讨论了这种新兴细菌如何利用许多这些sRNA来适应其生态位并成为成功的人类病原体。
