Huizhou Third people's hospital, Guangzhou medical university, Huizhou, 516002, Guangdong, P.R. China.
Department of Radiology, the Second Affiliated Hospital, School of Medicine, South China University of Technology, 516002, Guangdong, P.R. China.
Cell Biochem Biophys. 2023 Mar;81(1):7-17. doi: 10.1007/s12013-022-01124-x. Epub 2023 Jan 11.
Ferroptosis is a newfound mode of regulated cell death that may have potential to associate with prognostic or diagnostic factors in glioma. In this research, 5 genes related to glioma were screened through the FerrDb database, and we analyzed the combination between genes and glioma of survival and prognosis via TCGA, GEPIA, TIMER, and other databases. Survival curve and prognostic analysis showed that the overexpression of NFE2L2 and NOX4, respectively, has a remarkable link with a worse prognosis in glioma. Then, the association between the expression of the two genes and tumor-infiltrating immune cells level was explored based on the GSCA, and the immunity of NFE2L2 and NOX4 based on the TISIDB database was also investigated. In glioma, especially GBM, there is a strong association between gene expression and immune infiltration, even in macrophages, nTreg, and Th2 cells, which play immunosuppressive functions in TME. In conclusion, these results indicate that NFE2L2 and NOX4 could be risk prognosis biomarkers in glioma, and they bound up with immune infiltration and tumor immunity in tumorigenesis.
铁死亡是一种新发现的细胞程序性死亡方式，可能与胶质瘤的预后或诊断因素有关。在这项研究中，我们通过 FerrDb 数据库筛选出与胶质瘤相关的 5 个基因，并通过 TCGA、GEPIA、TIMER 等数据库分析基因与胶质瘤的生存和预后的关系。生存曲线和预后分析表明，NFE2L2 和 NOX4 的过表达分别与胶质瘤的预后不良显著相关。然后，基于 GSCA 探讨了这两个基因的表达与肿瘤浸润免疫细胞水平之间的关联，并基于 TISIDB 数据库研究了 NFE2L2 和 NOX4 的免疫情况。在胶质瘤中，特别是在 GBM 中，基因表达与免疫浸润之间存在很强的关联，甚至在巨噬细胞、nTreg 和 Th2 细胞中也是如此，这些细胞在 TME 中发挥免疫抑制作用。总之，这些结果表明，NFE2L2 和 NOX4 可能是胶质瘤的风险预后生物标志物，它们与肿瘤发生中的免疫浸润和肿瘤免疫有关。
