School of Materials and Chemistry, University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai 200093, PR China; College of Chemistry and Chemical Engineering, Wuhan Textile University, No. 1 Yangguang Avenue, Wuhan 430200, PR China.
Department of Gastroenterology, Changhai Hospital, Naval Medical University, No. 168 Changhai Road, Shanghai 200433, PR China.
Int J Biol Macromol. 2023 Mar 1;230:123196. doi: 10.1016/j.ijbiomac.2023.123196. Epub 2023 Jan 10.
The postoperative recurrence has adversely affected the treatment of tumors. Besides, the potential bacterial infection at the wound site may lead to a series of tissue necrosis. Here, we developed an injectable γ-polyglutamic acid/carboxymethyl chitosan/polydopamine hydrogel (PCP) for simultaneously reducing the postoperative infection and preventing the tumor recurrence. On the one hand, the aqueous solution of carboxymethyl chitosan oxidized the dopamine into polydopamine; on the other, the carboxymethyl chitosan was cross-linked with the activated γ-polyglutamic acid to form a hydrogel. After local implantation, the PCP hydrogel effectively killed tumor cells and bacteria under 808 nm laser irradiation. In addition, carboxymethyl chitosan rendered the hydrogel with anti-bacterial properties as well as anti-tumor efficiencies. The anti-tumor recurrence and anti-bacterial efficiencies of PCP hydrogel were proved on a tumor-removed mouse model and a Staphylococcus aureus-infected mouse model, respectively. Moreover, the hydrogel has the advantages of good biocompatibility and simple preparation, and thus has potential application prospects in the prevention of tumor recurrence and wound bacterial infection.
术后复发对肿瘤的治疗产生了不利影响。此外，伤口部位的潜在细菌感染可能导致一系列组织坏死。在这里，我们开发了一种可注射的γ-聚谷氨酸/羧甲基壳聚糖/聚多巴胺水凝胶（PCP），用于同时减少术后感染和预防肿瘤复发。一方面，氧化羧甲基壳聚糖将多巴胺氧化成聚多巴胺；另一方面，羧甲基壳聚糖与激活的γ-聚谷氨酸交联形成水凝胶。局部植入后，PCP 水凝胶在 808nm 激光照射下能有效杀死肿瘤细胞和细菌。此外，羧甲基壳聚糖使水凝胶具有抗菌性能和抗肿瘤效率。PCP 水凝胶的抗肿瘤复发和抗菌效率分别在肿瘤切除小鼠模型和金黄色葡萄球菌感染小鼠模型中得到了验证。此外，该水凝胶具有良好的生物相容性和简单的制备优点，因此在预防肿瘤复发和伤口细菌感染方面具有潜在的应用前景。
