Chen Xinqi, Pan Ting, Guo Guohu, Chen Guohao, Cai Yongchang, Tang Yuxin, Wang Yijun, Wang Yucheng, Deng Zhenwei, Li Libo, Zhou Yan
Department of General Surgery, Affiliated Dongguan Hospital, Southern Medical University, Dongguan, China.
Department of Blood Transfusion, Affiliated Dongguan Hospital, Southern Medical University, Dongguan, China.
J Gastrointest Oncol. 2022 Dec;13(6):2832-2844. doi: 10.21037/jgo-22-1014.
Long non-coding ribonucleic acids (lncRNAs) are a class of non-coding RNAs implicated in the development of many malignancies, including gastric cancer (GC). In this study, we investigated the functions and molecular mechanisms of non-coding RNA activated by deoxyribonucleic acid damage () in GC.
expression at the messenger RNA levels was determined by quantitative reverse transcriptase (RT)-polymerase chain reaction assays. Cell proliferation, migration, and invasion were detected by Cell Counting Kit-8 assays, tumor formation assays, and Transwell assays. Cell-cycle distribution was detected by a flow cytometry analysis. location was detected by nucleocytoplasmic fractionation assays. The interaction between and the ()/ () axis was verified by dual-luciferase reporter gene assays and RNA binding protein immunoprecipitation (RIP) assays. Western blot was used to study the phosphatase and tensin homolog ()/phosphoinositide 3-kinases ()/protein kinase B () signaling pathway.
NORAD was upregulated in the GC tissues and cell lines. The silencing of repressed cell proliferation and the Growth 2 (G2)/Mitosis (M) cell-cycle transition in GC. also regulated expression by targeting and mediated signaling in GC.
We found that acts as an oncogene in GC. Our findings might provide a novel therapeutic target for GC.
长链非编码核糖核酸（lncRNAs）是一类非编码RNA，与包括胃癌（GC）在内的许多恶性肿瘤的发生发展有关。在本研究中，我们探讨了DNA损伤激活的非编码RNA（）在GC中的功能及分子机制。
通过定量逆转录（RT）-聚合酶链反应检测信使RNA水平的表达。采用细胞计数试剂盒-8检测、肿瘤形成检测和Transwell检测来检测细胞增殖、迁移和侵袭。通过流式细胞术分析检测细胞周期分布。通过核质分离检测定位。通过双荧光素酶报告基因检测和RNA结合蛋白免疫沉淀（RIP）检测验证与磷酸酶和张力蛋白同源物（）/磷脂酰肌醇3激酶（）/蛋白激酶B（）信号轴之间的相互作用。采用蛋白质免疫印迹法研究该信号通路。
在GC组织和细胞系中上调。沉默可抑制GC细胞的增殖以及生长2（G2）/有丝分裂（M）细胞周期转换。还通过靶向调节表达，并在GC中介导信号传导。
我们发现作为GC中的一种癌基因。我们的研究结果可能为GC提供一个新的治疗靶点。
