Liu Shuang-Shuang, Wu Wei-Na, Zhao Xiao-Lei, Fan Yun-Chang, Wang Yuan, Xu Zhi-Hong
College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo, 454000, PR China.
College of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo, 454000, PR China.
Anal Chim Acta. 2023 Mar 8;1245:340867. doi: 10.1016/j.aca.2023.340867. Epub 2023 Jan 20.
The microenvironments of biological systems are associated with the pathology of organisms. This study, aimed to construct a hemicyanine-based probe (1), which can respond to mitochondrial viscosity and hydrazine (NH), for imaging application in living cells and zebrafish. The probe showed no fluorescence due to the intramolecular rotation in the solution; however, it exhibited a strong emission at 730 nm when the molecules were restricted to a high-viscosity environment. The addition of NH caused an elimination reaction of the N-substituted group in the pyridinium part and further broke the CC bond to produce a highly fluorescent hydrazone. Also, the probe could selectively and quantitatively detect NH via the fluorescence enhancement at 510 nm in a concentration range of 0 μM-140μM, with the limit of detection being 0.0485 μM. This probe may be used to study diseases related to NH and viscosity changes in biological systems. Furthermore, the analysis methods based on probe 1 for NH detection in soil, water, and air samples were successfully established.
生物系统的微环境与生物体的病理学相关。本研究旨在构建一种基于半花菁的探针（1），其能够响应线粒体粘度和肼（NH），用于活细胞和斑马鱼的成像应用。该探针在溶液中由于分子内旋转而无荧光；然而，当分子被限制在高粘度环境中时，它在730nm处表现出强烈发射。加入NH会导致吡啶部分的N-取代基团发生消除反应，并进一步断裂C-C键以产生高荧光的腙。此外，该探针能够在0μM-140μM浓度范围内通过510nm处的荧光增强选择性地定量检测NH，检测限为0.0485μM。该探针可用于研究与NH和生物系统中粘度变化相关的疾病。此外，还成功建立了基于探针1检测土壤、水和空气样品中NH的分析方法。
