Zhang Yanyan, Song Yongqi, Zhang Wenchang, Xiao Tianfang, Peng Hui
College of Animal Science and Technology, Hainan University, Haikou, Hainan, China.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Anim Biotechnol. 2023 Dec;34(9):4547-4552. doi: 10.1080/10495398.2023.2166840. Epub 2023 Jan 18.
NLR family pyrin domain containing 9 () is a mammalian reproduction-related gene. In this study, we researched the associations between polymorphisms located in the coding sequence (CDS) of the gene, and both the total number of piglets born per litter (TNB) and the number of piglets born alive per litter (NBA) in Canada Large White pigs (CLW). We detected a single nucleotide polymorphism (SNP) within exon 3 (g.10910C > T). The allele frequencies at the locus were 0.474 for the C allele and 0.526 for the T allele. Three genotypes, CC, CT, and TT, occurred with frequencies of 0.216, 0.515, and 0.269, respectively. Sows with the CC genotype had the largest TNB and NBA, sows with TT had the smallest, and those with CT were in-between. This difference was statistically significant ( < 0.05). Furthermore, CC females grew faster than CT or TT females, and there was a significant relationship between polymorphism and the average daily gain ( < 0.05). Here, we provide the first evidence for a novel SNP in associated with litter size in CLW sows, which could be used as a genetic marker to improve litter size in pig breeding and production.
含吡啉结构域的NLR家族成员9（）是一个与哺乳动物繁殖相关的基因。在本研究中，我们研究了该基因编码序列（CDS）中的多态性与加拿大大白猪（CLW）每窝产仔总数（TNB）和每窝活产仔数（NBA）之间的关联。我们在第3外显子内检测到一个单核苷酸多态性（SNP）（g.10910C>T）。该位点的等位基因频率，C等位基因为0.474，T等位基因为0.526。三种基因型CC、CT和TT的出现频率分别为0.216、0.515和0.269。CC基因型的母猪TNB和NBA最大，TT基因型的母猪最小，CT基因型的母猪介于两者之间。这种差异具有统计学意义（<0.05）。此外，CC基因型的母猪比CT或TT基因型的母猪生长速度更快，且该基因多态性与平均日增重之间存在显著关系（<0.05）。在此，我们首次提供证据表明，CLW母猪中该基因的一个新型SNP与产仔数相关，可作为猪育种和生产中提高产仔数的遗传标记。
