一种来自金顶侧耳的新型真菌病毒的全基因组序列

Complete genome sequence of a novel mycovirus from Pleurotus citrinopileatus.

作者信息

Li Xuefei, Li Shiyu, Yin Weiqi, Sossah Frederick Leo, Song Bing, Li Yu, Zhang Bo

机构信息

Joint International Research Laboratory of Modern Agricultural Technology, Ministry of Education, Jilin Agricultural University, 130118, Changchun, China.

Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, 130118, Changchun, Jilin, China.

出版信息

Arch Virol. 2023 Jan 19;168(2):66. doi: 10.1007/s00705-022-05668-4.

DOI:10.1007/s00705-022-05668-4

PMID:36653596

Abstract

The complete genome sequence of a novel single-stranded [+ ssRNA] positive-sense (+) RNA mycovirus, designated as "Pleurotus citrinopileatus ourmiavirus 1" (PcOV1), isolated from Pleurotus citrinopileatus strain CCMJ2141, was determined. The complete genome of PcOV1 is composed of 2,535 nucleotides. It contains a single open reading frame (ORF), which encodes a protein of 657 amino acids (aa) containing conserved domains of an RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis based on RdRp sequences revealed that PcOV1 is a new member of the genus Ourmiavirus in the family Botourmiaviridae. This is the first virus from P. citrinopileatus to be characterized.

摘要

从金顶侧耳菌株CCMJ2141中分离出一种新型单链[+ ssRNA]正义（+）RNA真菌病毒，命名为“金顶侧耳欧尔密病毒1”（PcOV1），并测定了其全基因组序列。PcOV1的全基因组由2535个核苷酸组成。它包含一个单一的开放阅读框（ORF），编码一个657个氨基酸（aa）的蛋白质，该蛋白质含有RNA依赖的RNA聚合酶（RdRp）的保守结构域。基于RdRp序列的系统发育分析表明，PcOV1是布托欧尔密病毒科欧尔密病毒属的一个新成员。这是首个被鉴定的来自金顶侧耳的病毒。