Atatürk Public Hospital, Pathology, Antalya, Turkey.
Department of Pathology, Medical Faculty, Osmangazi University, Eskişehir, Turkey.
Indian J Pathol Microbiol. 2023 Jan-Mar;66(1):31-37. doi: 10.4103/ijpm.ijpm_814_21.
The programmed death-1 (PD-1) is an immune checkpoint molecule that suppresses T-cell response. The binding of PD-1 to PD-L1/PD-L2 results cytokine production, and T-cell proliferation are reduced. Tumors expressing PD-L1 and PD-L2 escape from cytotoxic T-cells and are exposed to tumor progression. For this reason, immunotherapy has become a new option in the treatment of cancer.
In this study, we examined the PD-L1 and PD-L2 expression in colorectal carcinoma (CRC), and evaluated the relationship between clinicopathological parameters and CD8+ T cells.
We evaluated CD8 expression in tumor-infiltrating lymphocytes and surrounding tumor lymphocytes with PD-L1, PD-L2 staining in tumor cells and immune cells formalin-fixed paraffin embedded samples of 124 patient diagnosed with CRC.
Pearson Chi-Square, Fisher Exact Chi-Square, and Pearson Exact Chi-Square analyses were used in the analysis of the cross tables. Survival distributions predicted Kaplan--Meier method and it was evaluated using log-rank statistics.
In our study, a significant correlation was found between PD-L1 expression and female sex and tumors with medullary morphology. No expression of PD-L2 was observed in tumors containing medullary morphology, and a statistically inverse relationship was observed between PD-L2 and the medullary component. PD-L1 positive tumor-infiltrating lymphocytes were determined to be an important predictor for recurrence-free survival.
We believe that the evaluation of these parameters may be useful in the selection of patients who will benefit from immunotherapy in CRC cases.
程序性死亡受体-1（PD-1）是一种免疫检查点分子，可抑制 T 细胞反应。PD-1 与 PD-L1/PD-L2 的结合导致细胞因子产生，T 细胞增殖减少。表达 PD-L1 和 PD-L2 的肿瘤逃避细胞毒性 T 细胞的攻击，从而暴露于肿瘤进展中。因此，免疫疗法已成为癌症治疗的新选择。
本研究检测了结直肠癌（CRC）中 PD-L1 和 PD-L2 的表达，并评估了其与临床病理参数和 CD8+T 细胞之间的关系。
我们评估了 124 例经组织学诊断为 CRC 的患者的肿瘤浸润淋巴细胞和周围肿瘤淋巴细胞中 CD8 的表达，以及肿瘤细胞和免疫细胞中 PD-L1、PD-L2 的染色情况。
采用 Pearson Chi-Square、Fisher Exact Chi-Square 和 Pearson Exact Chi-Square 分析交叉表。采用 Kaplan-Meier 方法预测生存分布，并采用对数秩检验进行评估。
在我们的研究中，PD-L1 的表达与女性和具有髓样形态的肿瘤之间存在显著相关性。具有髓样形态的肿瘤中未观察到 PD-L2 的表达，并且 PD-L2 与髓样成分之间存在统计学上的负相关关系。PD-L1 阳性肿瘤浸润淋巴细胞被确定为无复发生存的重要预测因子。
我们认为，评估这些参数可能有助于选择将从 CRC 免疫治疗中获益的患者。
