植物油对ARPE - 19细胞摄取叶黄素和玉米黄质的影响比较。
Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells.
作者信息
Baek Jeonghun, Mai Chun Wai, Lim Wei Meng, Wong Lai Chun
机构信息
School of Medicine, International Medical University, Kuala Lumpur 57000, Malaysia.
Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, Kuala Lumpur 57000, Malaysia.
出版信息
Int J Ophthalmol. 2023 Jan 18;16(1):40-46. doi: 10.18240/ijo.2023.01.06. eCollection 2023.
AIM
To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial (ARPE)-19 cells .
METHODS
ARPE-19 cells were cultured in Dulbecco's Modified Eagle Medium-F-12 supplemented with 10% foetal bovine serum and 1% penicillin-streptomycin in a humidified 5% CO incubator maintained at 37°C. Cells were treated with 247 µmol/L lutein, 49 µmol/L zeaxanthin and 1% (v/v) of either coconut oil, corn oil, peanut oil, olive oil, sunflower oil, soybean oil, castor oil, or linseed oil for 48h. Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography.
RESULTS
Among the oils tested, the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil.
CONCLUSION
ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils.
目的
比较植物油对成人视网膜色素上皮（ARPE）-19细胞摄取叶黄素和玉米黄质的影响。
方法
将ARPE-19细胞培养于补充有10%胎牛血清和1%青霉素-链霉素的杜尔贝科改良伊格尔培养基-F-12中，置于37°C、5%二氧化碳饱和湿度的培养箱中。用247µmol/L叶黄素、49µmol/L玉米黄质和1%（体积/体积）的椰子油、玉米油、花生油、橄榄油、向日葵油、大豆油、蓖麻油或亚麻籽油处理细胞48小时。通过高效液相色谱法定量细胞中叶黄素和玉米黄质的浓度。
结果
在所测试的油中，椰子油对叶黄素和玉米黄质的摄取量最高，而亚麻籽油的摄取量最低。
结论
发现ARPE-19对叶黄素和玉米黄质的摄取取决于油的类型。
Zotero 插件