Cuvelier C A, Morson B C, Roels H J
Department of Pathology, State University Ghent, Belgium.
Histopathology. 1987 Sep;11(9):927-39. doi: 10.1111/j.1365-2559.1987.tb01899.x.
The nuclear DNA content was determined with a cytophotometric technique in colonic mucosa of 15 patients with long-standing ulcerative colitis. The epithelial lesions were classified into inactive colitis, low grade and high grade dysplasia and adenocarcinoma. The histogram pattern varied between narrow unimodal in quiescent colitis, broad unimodal in low grade dysplasia with some hypertetraploid values in three cases (27%) and aneuploid in 62.5% of the lesions with high grade dysplasia. In well-differentiated adenocarcinoma the histograms were broad unimodal, whereas the curves of moderately and poorly differentiated carcinomas were wider and aneuploid. The technique can be used for a prognostic purpose: in dysplastic lesions, the detection of aneuploidy is important because it is frequently found in the presence of invasion although it does not allow its prediction. Carcinomas with polyploid DNA distribution have a better outcome than tumours with aneuploid distribution.
采用细胞光度测定技术测定了15例长期溃疡性结肠炎患者结肠黏膜的核DNA含量。上皮病变分为非活动性结肠炎、低级别和高级别发育异常及腺癌。直方图模式在静止期结肠炎时为窄单峰，低级别发育异常时为宽单峰，3例（27%）有一些超四倍体值，高级别发育异常病变中有62.5%为非整倍体。在高分化腺癌中，直方图为宽单峰，而中分化和低分化癌的曲线更宽且为非整倍体。该技术可用于预后评估：在发育异常病变中，非整倍体的检测很重要，因为它虽不能预测侵袭，但在侵袭存在时经常被发现。具有多倍体DNA分布的癌比具有非整倍体分布的肿瘤预后更好。
